Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Structural analysis of a fiber-pseudotyped adenovirus with ocular tropism suggests differential modes of cell receptor interactions
Autore:
Chiu, CY; Wu, E; Brown, SL; Von Seggern, DJ; Nemerow, GR; Stewart, PL;
Indirizzi:
Scripps Clin & Res Inst, Dept Immunol, La Jolla, CA 92037 USA Scripps Clin& Res Inst La Jolla CA USA 92037 nol, La Jolla, CA 92037 USA Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA90095 USA Univ Calif Los Angeles, Sch Med, Crump Inst Mol Imaging, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 11, volume: 75, anno: 2001,
pagine: 5375 - 5380
SICI:
0022-538X(200106)75:11<5375:SAOAFA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBGROUP-C; ELECTRON-MICROSCOPY; ALPHA(V) INTEGRINS; SIALIC-ACID; BINDING; PROTEIN; RESOLUTION; TYPE-37; DOMAIN; VECTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Nemerow, GR Scripps Clin & Res Inst, Dept Immunol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 a, CA 92037 USA
Citazione:
C.Y. Chiu et al., "Structural analysis of a fiber-pseudotyped adenovirus with ocular tropism suggests differential modes of cell receptor interactions", J VIROLOGY, 75(11), 2001, pp. 5375-5380

Abstract

Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes, Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis inhumans, does not infect cells via CAR despite demonstrated binding of the Ad37 knob to CAR. We have pseudotyped a fiber deletion Ad5 vector with the Ad37 fiber (Ad37f), and this vector retains the ocular tropism of Ad37, Here we present a cryo-electron microscopy reconstruction of Ad37f that shows the entire Ad37 fiber, including the shaft and knob domains. We have previously proposed that Ad37 may not utilize CAR for cell entry because of the geometric constraints imposed by a rigid fiber (E. Wu, J, Fernandez, S, K, Fleck, D, Von Seggern, S, Huang, and G, R, Nemerow, Virology 279:78-89, 2001), Consistent with this hypothesis, our structural results show that the Ad37 fiber is straight acid rigid, Modeling of the interaction between Ad37f and host cell receptors indicates that fiber flexibility or rigidity, as well as length, can affect receptor usage and cellular tropism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 14:59:57