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Titolo:
REDUCING MEDIUM FOR THE CULTIVATION OF PORPHYROMONAS-GINGIVALIS
Autore:
PEDERSON ED; TURNER DW; LAMBERTS BL; SCHADE SZ;
Indirizzi:
USN,DENT RES INST,2701 SHERIDAN RD,BLDG 1-H,NAVL TRAINING CTR GREAT LAKES IL 60088
Titolo Testata:
Microbios
fascicolo: 359, volume: 89, anno: 1997,
pagine: 119 - 124
SICI:
0026-2633(1997)89:359<119:RMFTCO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; VIRULENCE; W50;
Keywords:
REDUCING MEDIUM; PORPHYROMONAS GINGIVALIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
E.D. Pederson et al., "REDUCING MEDIUM FOR THE CULTIVATION OF PORPHYROMONAS-GINGIVALIS", Microbios, 89(359), 1997, pp. 119-124

Abstract

Porphyromonas gingivalis, a micro-organism frequently associated withhuman periodontal diseases, is commonly cultured under a reducing atmosphere in enclosed cabinets or glove boxes. This paper reports a modified Wilkins-Chalgren (MWC) medium for the culture of Fl gingivalis under atmospheric conditions at 37 degrees C. On the basis of preliminary tests, WC broth was supplemented as follows: 500 mg/l cysteine hydrochloride; 250 mg/l sodium thioglycolate; and 1,000 mg/l sodium bicarbonate. Three Fl gingivalis isolates (JKG-I, 33277 and A7436) showed very similar growth over 24 and 48 h periods when cultured both in WC medium in an anaerobic chamber and in the MWC medium. Culture of these isolates both in the anaerobic chamber and in the MWC medium yielded very similar data with respect to trypsin-like activity, total protease activity, and reactivity to monoclonal antibodies specific for P. gingivalis. Growth in the MWC medium varied over a 3- to 4-fold range for seven additional isolates (JKG-7, D86B6, D13B11, D84D2, JKG9, D67D9, D82F5) over 24 and 48 h periods.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:56:33