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Titolo:
Development of a tyrosinase-based, screen-printed amperometric electrode for the detection of flavanoid polyphenols in lager beers
Autore:
Cummings, EA; Eggins, BR; McAdams, ET; Linquette-Mailley, S; Mailley, P; Madigan, D; Clements, M; Coleman, C;
Indirizzi:
Univ Ulster, No Ireland Bio Engn Ctr, Newtownabbey BT37 0QB, Antrim, NorthIreland Univ Ulster Newtownabbey Antrim North Ireland BT37 0QB trim, NorthIreland Univ Ulster, Sch Appl Biol & Chem Sci, Newtownabbey BT37 0QB, Antrim, North Ireland Univ Ulster Newtownabbey Antrim North Ireland BT37 0QB rim, North Ireland CEA, DRFMC, F-38054 Grenoble, France CEA Grenoble France F-38054CEA, DRFMC, F-38054 Grenoble, France Guinness Ireland Grp, Dublin 8, Ireland Guinness Ireland Grp Dublin Ireland 8 ess Ireland Grp, Dublin 8, Ireland
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
fascicolo: 2, volume: 59, anno: 2001,
pagine: 84 - 89
SICI:
0361-0470(2001)59:2<84:DOATSA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ULTRAVIOLET DETECTION METHODS; CARBON-PASTE BIOSENSOR; PROANTHOCYANIDINS; PHENOLS; BARLEY; SENSOR; TISSUE;
Keywords:
biosensor; catechol; (+)-catechin; (-)-epicatechin; flow injection analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Cummings, EA Univ Ulster, No Ireland Bio Engn Ctr, Shore Rd, Newtownabbey BT37 0QB, Antrim, North Ireland Univ Ulster Shore Rd Newtownabbey Antrim North Ireland BT37 0QB
Citazione:
E.A. Cummings et al., "Development of a tyrosinase-based, screen-printed amperometric electrode for the detection of flavanoid polyphenols in lager beers", J AM SOC BR, 59(2), 2001, pp. 84-89

Abstract

Amphiphilic, tyrosinase-modified screen-printed carbon bioelectrodes were fabricated and compared to the p-dimethyaminocinnamaldehyde (DAC) colorimetric method for the analysis of flavanoid polyphenols in authentic lager beer samples. Initially, the performances of the biosensors under flowing conditions were appraised using catechol as a model substrate. The electrodes displayed rapid response times and a high degree of sensitivity and reproducibility upon injection of catechol onto a single manifold. In addition, simple flavanols, separated from barley, were utilized to assess the sensitivity of the biosensors afforded by the presence of the enzyme. The bioelectrode sensitivity decreased upon an increase in molecule size. Finally, using flow injection analysis, authentic beer samples were analyzed using the biosensor and compared to the DAC colorimetric method. A good correlation between the two methods of analysis was observed. However, due to the lack of enzyme substrate specificity, the biosensor response did not decrease to thesame extent as the colorimetric method; this can be attributed to the presence of interferents, e.g., ferulic acid and p-coumaric acid.

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Documento generato il 22/01/20 alle ore 12:34:25