Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Quantitation of suspensions (MESED). Application of MESED-GC/MS in the quantitation of ifosfamide mustard in erythrocytes, plasma, and plasma water
Autore:
Dumez, H; Guetens, G; De Boeck, G; Highley, M; Tjaden, UR; Maes, R; Hanauske, A; Van Oosterom, AT; de Bruijn, EA;
Indirizzi:
Katholieke Univ Leuven, UZ Gasthuisberg, Expt Oncol Lab, Dept Clin Oncol, B-3000 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3000 l, B-3000 Louvain, Belgium Univ Utrecht, Utrech Inst Pharmaceut Sci, Dept Toxicol, Utrecht, Netherlands Univ Utrecht Utrecht Netherlands ci, Dept Toxicol, Utrecht, Netherlands Leiden Univ, Div Biopharmaceut Sci, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands Ninewells Hosp, Dept Canc Med, Dundee DD1 9SY, Scotland Ninewells Hosp Dundee Scotland DD1 9SY anc Med, Dundee DD1 9SY, Scotland Univ Instelling Antwerp, Dept Chem, B-2610 Wilrijk, Belgium Univ Instelling Antwerp Wilrijk Belgium B-2610 , B-2610 Wilrijk, Belgium
Titolo Testata:
JOURNAL OF SEPARATION SCIENCE
fascicolo: 2, volume: 24, anno: 2001,
pagine: 123 - 128
SICI:
1615-9314(200102)24:2<123:QOS(AO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Keywords:
sample pretreatment; suspension; sediment; flow resistance; red blood cells/erythrocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
2
Recensione:
Indirizzi per estratti:
Indirizzo: de Bruijn, EA Katholieke Univ Leuven, UZ Gasthuisberg, Expt Oncol Lab, Dept Clin Oncol, Herestr 49, B-3000 Louvain, Belgium Katholieke Univ Leuven Herestr 49 Louvain Belgium B-3000 um
Citazione:
H. Dumez et al., "Quantitation of suspensions (MESED). Application of MESED-GC/MS in the quantitation of ifosfamide mustard in erythrocytes, plasma, and plasma water", J SEP SCI, 24(2), 2001, pp. 123-128

Abstract

Compounds associated with particles in suspension are difficult to quantitate in the analysis of drugs in red blood cells. Breakthrough experiments were performed for the determination of a mustard gas related substance, ifosfamide mustard, originating from the anticancer agent ifosfamide. It is shown that the use of the measurement of sediment (MESED) instrument enables a comparison of ifosfamide mustard levels in red blood cells, total plasma,and plasma water. Red blood cell sediments were analysed by GC/MS togetherwith plasma and plasma water extracts. The MESED maintains a direct relationship between the volume and weight of red blood cell sediments without disturbance of the equilibrium of a substance between the cells and the plasma, the latter being the suspending fluid. The MESED GC/MS combination has been applied successfully in clinical settings, estimating the importance ofthe red blood cell, plasma, and plasma water fractions in the presentationof ifosfamide mustard to other tissues by the blood. The presence of ifosfamide and ifosfamide mustard in red blood cells, total plasma, and plasma water was confirmed by negative chemical ionisation (NCI) mass spectrometry of the trifluoroacetylated derivatives following capillary GC. The NCl-massspectra of ifosfamide, ifosfamide mustard, and the internal standard cyclophosphamide provide specific fragment ion masses, which can be used in single or double ion monitoring of the substances for quantitation of cancer patient samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:08:59