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Titolo:
Monocyte chemoattractant protein-1 expression by osteoblasts following infection with Staphylococcus aureus or Salmonella
Autore:
Bost, KL; Bento, JL; Petty, CC; Schrum, LW; Hudson, MC; Marriott, I;
Indirizzi:
Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA Univ N Carolina Charlotte NC USA 28223 Dept Biol, Charlotte, NC 28223 USA
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 5, volume: 21, anno: 2001,
pagine: 297 - 304
SICI:
1079-9907(200105)21:5<297:MCPEBO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-RESORPTION; ENDODONTIC INFECTIONS; CULTURED OSTEOBLASTS; ADOPTIVE TRANSFER; IN-VITRO; T-CELLS; MECHANISMS; MOUSE; MICE; INTERNALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Bost, KL Univ N Carolina, Dept Biol, 9201 Univ City Blvd, Charlotte, NC 28223 USA Univ N Carolina 9201 Univ City Blvd Charlotte NC USA 28223 23 USA
Citazione:
K.L. Bost et al., "Monocyte chemoattractant protein-1 expression by osteoblasts following infection with Staphylococcus aureus or Salmonella", J INTERF CY, 21(5), 2001, pp. 297-304

Abstract

Two common pathogens of bone, Staphylococcus aureus and Salmonella, were investigated for their ability to induce chemokine expression in bone-forming osteoblasts, Cultured mouse or human osteoblasts could rapidly respond tobacterial infection by upregulating the mRNA encoding the chemokine, monocyte chemoattractant protein-1 (MCP-1). This rapid induction occurred on infection with either the gram-positive pathogen, S. aureus, or the gram-negative pathogen, Salmonella, Increased mRNA expression translated into MCP-1 secretion by cultured mouse or human osteoblasts in response to viable bacteria, whereas UV-killed bacteria were less effective in stimulating chemokine secretion. There was a dose-response relationship observed between the amount of input bacteria and increases in MCP-1 secretion. Immunohistochemical staining of infected osteoblasts indicated that the majority of cells could express MCP-1, with some osteoblasts having a higher intensity of staining than others. Organ cultures of mouse calvaria (skullcap) bone showed increases in MCP-1 immunostaining following bacterial infection. The immunoreactive MCP-1 in infected calvaria localized to areas containing active osteoblasts. Taken together, these studies demonstrate a conserved osteoblast-derived MCP-1 response to two very different pathogens of bone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:04:48