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Titolo:
Geometry dependent two-dimensional heteronuclear multiplet effects in paramagnetic proteins
Autore:
Madhu, PK; Grandori, R; Hohenthanner, K; Mandal, PK; Muller, N;
Indirizzi:
Johannes Kepler Univ, Inst Chem, A-4040 Linz, Austria Johannes Kepler Univ Linz Austria A-4040 Inst Chem, A-4040 Linz, Austria
Titolo Testata:
JOURNAL OF BIOMOLECULAR NMR
fascicolo: 1, volume: 20, anno: 2001,
pagine: 31 - 37
SICI:
0925-2738(200105)20:1<31:GDTHME>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CROSS-CORRELATED RELAXATION; TRANSVERSE RELAXATION; NUCLEAR-RELAXATION; NMR-SPECTROSCOPY; MACROMOLECULES; INTERFERENCE; ASSIGNMENT; SPECTRA; SYSTEMS; PROTONS;
Keywords:
Curie spin relaxation; cross correlations; myoglobin; paramagnetic protein; TROSY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, N Johannes Kepler Univ, Inst Chem, Altenbergerstr 69, A-4040 Linz,Austria Johannes Kepler Univ Altenbergerstr 69 Linz Austria A-4040 tria
Citazione:
P.K. Madhu et al., "Geometry dependent two-dimensional heteronuclear multiplet effects in paramagnetic proteins", J BIOM NMR, 20(1), 2001, pp. 31-37

Abstract

We report experimental observation and numerical simulation of a two-dimensional multiplet effect in the heteronuclear correlation spectrum of a paramagnetic protein that depends on molecular geometry. This effect arises as a consequence of cross-correlated relaxation involving the Curie spin relaxation and internuclear dipolar relaxation mechanisms. It also manifests itself in resolution and sensitivity improvement in transverse relaxation optimised spectroscopy (TROSY) kind of experiments. Characteristic multiplet patterns in heteronuclear coupled two-dimensional NMR spectra encode directional information for the heteronuclear bond with respect to the paramagneticcenter. These patterns, which are simulated here using Redfield's relaxation theory, can be used to obtain a new type of geometry restriction for structure determination and refinement of paramagnetic macromolecular systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 14:54:49