Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase
Autore:
Sahlgren, CM; Mikhailov, A; Hellman, J; Chou, YH; Lendahl, U; Goldman, RD; Eriksson, JE;
Indirizzi:
Univ Turku, Dept Biol, Physiol Anim Lab, FIN-20014 Turku, Finland Univ Turku Turku Finland FIN-20014 ol Anim Lab, FIN-20014 Turku, Finland Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Biol, S-17177 Stockholm, Sweden Northwestern Univ, Sch Med, Dept Cell Mol & Struct Biol, Chicago, IL 60611USA Northwestern Univ Chicago IL USA 60611 Struct Biol, Chicago, IL 60611USA Russian Acad Med Sci, Canc Res Ctr, Moscow 115478, Russia Russian Acad MedSci Moscow Russia 115478 Res Ctr, Moscow 115478, Russia Abo Akad Univ, BioCity, Dept Biol, FIN-20520 Turku, Finland Abo Akad UnivTurku Finland FIN-20520 ept Biol, FIN-20520 Turku, Finland Univ Turku, Turku Ctr Biotechnol, FIN-20521 Turku, Finland Univ Turku Turku Finland FIN-20521 Biotechnol, FIN-20521 Turku, Finland
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 19, volume: 276, anno: 2001,
pagine: 16456 - 16463
SICI:
0021-9258(20010511)276:19<16456:MROTIF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASE-5; RHO-ASSOCIATED KINASE; MONOCLONAL-ANTIBODIES DISTINGUISH; HUMAN KERATIN-18; SKELETAL-MUSCLE; STEM-CELLS; VIMENTIN; IDENTIFICATION; EXPRESSION; MITOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Eriksson, JE Univ Turku, Dept Biol, Physiol Anim Lab, Sci Bldg 1, FIN-20014 Turku, Finland Univ Turku Sci Bldg 1 Turku Finland FIN-20014 Turku, Finland
Citazione:
C.M. Sahlgren et al., "Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase", J BIOL CHEM, 276(19), 2001, pp. 16456-16463

Abstract

The intermediate filament protein nestin is expressed during early stages of development in the central nervous system and in muscle tissues. Nestin expression is associated with morphologically dynamic cells, such as dividing and migrating cells. However, little is known about regulation of nestinduring these cellular processes. We have characterized the phosphorylation-based regulation of nestin during different stages of the cell cycle in a neuronal progenitor cell line, ST15A. Confocal microscopy of nestin organization and P-32 in vivo labeling studies show that the mitotic reorganization of nestin is accompanied by elevated phosphorylation of nestin. The phosphorylation-induced alterations in nestin organization during mitosis in ST15A cells are associated with partial disassembly of nestin filaments. Comparative in vitro and in vivo phosphorylation studies identified cdc2 as the primary mitotic kinase and Thr(316) as a cdc2-specific phosphorylation siteon nestin. We generated a phosphospecific nestin antibody recognizing the phosphorylated form of this site. By using this antibody we observed that nestin shows constitutive phosphorylation at Thr316, which is increased during mitosis. This study shows that nestin is reorganized during mitosis and that cdc2-mediated phosphorylation is an important regulator of nestin organization and dynamics during mitosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 17:21:04