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Titolo:
Calcium- and syntaxin 1-mediated trafficking of the neuronal glycine transporter GLYT2
Autore:
Geerlings, A; Nunez, E; Lopez-Corcuera, B; Aragon, C;
Indirizzi:
Univ Autonoma Madrid, Fac Ciencias, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 o Ochoa, E-28049 Madrid, Spain
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 20, volume: 276, anno: 2001,
pagine: 17584 - 17590
SICI:
0021-9258(20010518)276:20<17584:CAS1TO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPETITIVE ACTION-POTENTIALS; HUMAN DOPAMINE TRANSPORTER; SYNAPTIC VESICLES; NEUROTRANSMITTER RELEASE; NERVE-TERMINALS; PROLINE TRANSPORTER; MOLECULAR-BIOLOGY; PHORBOL ESTERS; PROTEIN; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Aragon, C Univ Autonoma Madrid, Fac Ciencias, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 -28049 Madrid, Spain
Citazione:
A. Geerlings et al., "Calcium- and syntaxin 1-mediated trafficking of the neuronal glycine transporter GLYT2", J BIOL CHEM, 276(20), 2001, pp. 17584-17590

Abstract

Previously we demonstrated the existence of a physical and functional interaction between the glycine transporters and the SNARE protein syntaxin 1, In the present report the physiological role of the syntaxin l-glycine transporter 2 (GLYT2) interaction has been investigated by using a brain-derived preparation. Previous studies, focused on syntaxin 1-transporter interactions using overexpression systems, led to the postulation that syntaxin is somehow implicated in protein trafficking. Since syntaxin 1 is involved in exocytosis of neurotransmitter and also interacts with GLYT2, we stimulatedexocytosis in synaptosomes and examined its effect on surface-expression and transport activity of GLYT2, We found that, under conditions that stimulate vesicular glycine release, GLYT2 is rapidly trafficked first toward theplasma membrane and then internalized. When the same experiments were performed with synaptosomes inactivated for syntaxin 1 by a pretreatment with the neurotoxin Bont/C, GLYT2 was unable to reach the plasma membrane but still was able to leave it. These results indicate the existence of a SNARE-mediated regulatory mechanism that controls the surface-expression of GLYT2. Syntaxin 1 is involved in the arrival to the plasma membrane but not in theretrieval. Furthermore, by using immunogold labeling on purified preparations from synaptosomes, we demonstrate that GLYT2 is present in small synaptic-like vesicles. GLYT2-containing vesicles may represent neurotransmitter transporter that is being trafficked. The results of our work suggest a close correlation between exocytosis of neurotransmitter and its reuptake by transporters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:16:48