Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Dimethyl sulfoxide affects the selection of splice sites
Autore:
Bolduc, L; Labrecque, B; Cordeau, M; Blanchette, M; Chabot, B;
Indirizzi:
Univ Sherbrooke, Fac Med, Dept Microbiol & Infectiol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 20, volume: 276, anno: 2001,
pagine: 17597 - 17602
SICI:
0021-9258(20010518)276:20<17597:DSATSO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; CELL-ADHESION MOLECULE; MURINE ERYTHROLEUKEMIA-CELLS; RETINOIC ACID; C-MYC; MOUSE ERYTHROLEUKEMIA; HNRNP A1; N-CAM; DIFFERENTIATION; DMSO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Chabot, B Univ Sherbrooke, Fac Med, Dept Microbiol & Infectiol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 PQ J1H 5N4, Canada
Citazione:
L. Bolduc et al., "Dimethyl sulfoxide affects the selection of splice sites", J BIOL CHEM, 276(20), 2001, pp. 17597-17602

Abstract

Depending on the cell lines and cell types, dimethyl sulfoxide (Me2SO) caninduce or block cell differentiation and apoptosis, Although Me2SO treatment alters many levels of gene expression, the molecular processes that are directly affected by Me2SO have not been clearly identified. Here, we report that Me2SO affects splice site selection on model pre-mRNAs incubated in a nuclear extract prepared from HeLa cells. A shift toward the proximal pair of splice sites was observed on pre-mRNAs carrying competing 5'-splice sites or competing 3'-splice sites. Because the activity of recombinant hnRNPAl protein was similar when added to extracts containing or lacking Me2SO,the activity of endogenous Al proteins is probably not affected by Me2SO. Notably, in a manner reminiscent of SR proteins, Me2SO activated splicing in a HeLa S100 extract. Moreover, the activity of recombinant SR proteins insplice site selection in vitro was improved by Me2SO, Polar solvents like DMF and formamide similarly modulated splice site selection in vitro but formamide did not activate a HeLa S100 extract. We propose that Me2SO improves ionic interactions between splicing factors that contain RS-domains, The direct impact of Me2SO on alternative splicing may explain, at least in part, the different and sometimes opposite effects of Me2SO on cell differentiation and apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:13:36