Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FGFR1, signaling, and AP-1 expression after retinal detachment: Reactive Muller and RPE cells
Autore:
Geller, SF; Lewis, GP; Fisher, SK;
Indirizzi:
Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 6, volume: 42, anno: 2001,
pagine: 1363 - 1369
SICI:
0146-0404(200105)42:6<1363:FSAAEA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; FOS GENE-EXPRESSION; NF-KAPPA-B; SUBFOVEAL CHOROIDAL NEOVASCULARIZATION; ACTIVATED PROTEIN-KINASE; RAT RETINA; EXTRACELLULAR SIGNALS; TRANSCRIPTION FACTOR; NEUROTROPHIC FACTOR; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Geller, SF Univ Sydney, F13, Sydney, NSW 2006, Australia Univ Sydney F13 Sydney NSW Australia 2006 , NSW 2006, Australia
Citazione:
S.F. Geller et al., "FGFR1, signaling, and AP-1 expression after retinal detachment: Reactive Muller and RPE cells", INV OPHTH V, 42(6), 2001, pp. 1363-1369

Abstract

PURPOSE. TO identify changes in cellular signaling pathways and AP-I expression in retina and retinal pigmented epithelium (RPE) after experimental retinal detachment (RD). METHODS. Cat and rabbit neural retinas were separated from the RPE in vivofor 5 minutes to 28 days. Tissues were removed and processed for Western blotting, immunohistochemistry, in situ hybridization, and immunoprecipitation experiments. RESULTS. An ordered sequence of events occurs after RD: (1) fibroblast growth factor (FGF) receptor 1 (FGFR1, flg) is phosphorylated in the retina within 15 minutes and dephosphorylated 2 hours after RD; (2) The extracellular signal-regulated kinase (ERK) is phosphorylated in both Muller and RPE cells within 15 minutes and remains so for several days; (3) De novo expression of c-fos mRNA coincides with increased c-Fos and c-Jun immunoreactivity in both Muller and RPE cells; (4) CREB is phosphorylated in a subpopulationof photoreceptors; and (5) STAT3 and NF-kappaB are activated in inner nuclear layer cells by 1 day of RD. CONCLUSIONS. These data suggest that nonneuronal cells (RPE and Muller cells) respond to RD very rapidly by stimulating ERK signaling and AP-1 transcription factor expression. Fur thermore, these data suggest that basic fibroblast growth factor (FGF-2, bFGF) is involved in initiating the retina's earliest responses to RD. The events described here precede changes in gene expression and morphology that can have serious effects on visual outcome in humans treated for retinal detachment or other retinal injuries.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 01:34:56