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Titolo:
Transforming growth factor alpha (TGF-alpha) increases cell number in a human pancreatic cancer cell line but not in normal mouse pancreas
Autore:
Kullenberg, B; Jansen, C; Fredang, N; Ohlsson, B; Axelson, J;
Indirizzi:
Univ Hosp MAS, Dept Surg, SE-20502 Malmo, Sweden Univ Hosp MAS Malmo Sweden SE-20502 S, Dept Surg, SE-20502 Malmo, Sweden Univ Lund, Dept Surg, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100Univ Lund, Dept Surg, S-22100 Lund, Sweden
Titolo Testata:
INTERNATIONAL JOURNAL OF PANCREATOLOGY
fascicolo: 3, volume: 28, anno: 2000,
pagine: 199 - 205
SICI:
0169-4197(200012)28:3<199:TGFA(I>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR RECEPTOR; PROLIFERATION; CARCINOMA; MICE; OVEREXPRESSION; GLAND;
Keywords:
human pancreatic cancer cell lines; LN-36; cell number; TGF-alpha; EGF; tyrphostin B56; XTT; mouse pancreas; weight; protein; DNA-synthesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Axelson, J Univ Hosp MAS, Dept Surg, SE-20502 Malmo, Sweden Univ Hosp MASMalmo Sweden SE-20502 g, SE-20502 Malmo, Sweden
Citazione:
B. Kullenberg et al., "Transforming growth factor alpha (TGF-alpha) increases cell number in a human pancreatic cancer cell line but not in normal mouse pancreas", INT J PANCR, 28(3), 2000, pp. 199-205

Abstract

Background. The pancreas harbors growth factors such as the epidermal growth factor (EGF) family. The physiological and pathophysiological roles of growth factors in normal pancreas remain unsettled. Human pancreatic cancer overexpresses the EGF receptor, and the ligands EGF and transforming growthfactor alpha (TGF-alpha). The aim of the present experiments was to study the effect of TGF-alpha in a pancreatic cancer cell line and in normal mouse pancreas. Method. The LN-36 cell line, established ft om a pancreatic duct cell adenocarcinoma, was incubated with TCF-alpha or EGF. The effect of an EGF receptor-specific, tyrosine kinase inhibitor (tyrphostin B56) with or without growth factors was also studied, The cell number was measured with the XTT-colorimetric method. TGF-alpha, the tyrphostins A25, B48, and B56, were in separate experiments infused during 1 wk to normal female mice by subcutaneous (sc) minipumps. Results. The LN-36 cell line responded to TGF-alpha, and EGF with increased cell number; +61% with 10(-10) M TGF-alpha and +34% with 10(-9) d EGF. Tyrphostin B56 at a concentration of 10(-5) M reduced the cell number by 76%,but when incubated together with growth factors the reduction was only 44%with TGF-alpha, and 39% with EGF. Infusion of TGF-alpha increased mouse pancreatic wet weight and protein content but was without effect on DNA synthesis, measured as incorporation of tritiated thymidine. Infusion of three different tyrphostins did not influence mice pancreas. Conclusion. The results support the role of TGF-alpha to maintain growth of pancreatic cancer cells bg the EGF receptor. Infusion of TGF-alpha induced hypertrophy in normal mouse pancreas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 11:13:05