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Titolo:
Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum)
Autore:
Yoon, TJ; Yoo, YC; Kang, TB; Her, E; Kim, SH; Kim, K; Azuma, I; Kim, JB;
Indirizzi:
Han Dong Univ, Inst Biomed Res, Buk Ku, Pohang 791940, Kyungbook, South Korea Han Dong Univ Pohang Kyungbook South Korea 791940 Kyungbook, South Korea Konyang Univ, Coll Med, Dept Microbiol, Nonsan 320711, Chungnam, South Korea Konyang Univ Nonsan Chungnam South Korea 320711 11, Chungnam, South Korea Kon Kuk Univ, Coll Med, Dept Immunol, Chung Ju 380701, South Korea Kon KukUniv Chung Ju South Korea 380701 l, Chung Ju 380701, South Korea Kyunghee Univ, Grad Sch E W Med Sci, Seoul 130701, South Korea Kyunghee Univ Seoul South Korea 130701 ed Sci, Seoul 130701, South Korea Capro Biotech, Biotechnol Innovat Ctr 406, Chunchon 200200, Kangwon Do, South Korea Capro Biotech Chunchon Kangwon Do South Korea 200200 gwon Do, South Korea Hakodate Natl Coll Technol, Hakodate, Hokkaido, Japan Hakodate Natl Coll Technol Hakodate Hokkaido Japan date, Hokkaido, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 5, volume: 1, anno: 2001,
pagine: 881 - 889
SICI:
1567-5769(200105)1:5<881:CAHAAO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURAMYL DIPEPTIDE DERIVATIVES; GALACTOSIDE-SPECIFIC LECTIN; AMINO-ACID-SEQUENCE; COLORATUM EXTRACT; FUTURE-PROSPECTS; CYTO-TOXICITY; TUMOR-CELLS; A-CHAIN; B-CHAIN; CYTOTOXICITY;
Keywords:
Korean mistletoe; lectin; adjuvant; vaccine; immune responses;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, JB Han Dong Univ, Inst Biomed Res, Buk Ku, Namsong Ri 3, Pohang 791940, Kyungbook, South Korea Han Dong Univ Namsong Ri 3 Pohang Kyungbook SouthKorea 791940 rea
Citazione:
T.J. Yoon et al., "Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum)", INT IMMUNO, 1(5), 2001, pp. 881-889

Abstract

The adjuvant effect of lectins (KML-C) isolated from Korean mistletoe (Viscum album coloratum) on induction of humoral and cellular immune responses against keyhole limpet hemocyanine (KLH) was examined. When mice were immunized subcutaneously (s.c.) with KLH (20 mug/mouse) admired with or without 50 ng/mouse of KML-C (KLH + KML-C), mice immunized with KLH + KML-C showed significantly higher antibody titers against KLH than those immunized with KLH alone. showing the highest titer 5 weeks after immunization. Furthermore, boost immunization with KLH + KML-C at 2-week interval elicited much higher activity than single immunization to enhance antibody responses againstKLH. The assay for determining isotypes of antibodies revealed that KML-C augmented KLH-specific antibody titers of IgG1, IgG2a and IgG2b. The culture supernatants obtained from the splenocytes of mice treated with KLH + KML-C also showed a higher level of both KLH-specific Th-l (IL-2 and IFN-gamma) and Th-2 type cytokine (IL-1). In an in vitro analysis of T lymphocyte proliferation to KLH on week 4, the splenocytes of mice treated with KLH + KML-C showed a significantly higher proliferating activity than those treatedwith KLH alone, In addition, mice immunized twice with KLH + KML-C and followed by intrafootpad (i.f.) injection of KLH (50 mug/site) 14 weeks after the primary immunization induced a higher delayed-type hypersensitivity (DTH) reaction than mice treated with KLH alone. These results suggest that KML-C is a potent immunoadjuvant to enhance cellular and humoral immune responses. (C) 2001 Elsevier Science B.V. All nights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:20:36