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Titolo:
The 3 ' Ig kappa enhancer contains RNA polymerase II promoters: implications for endogenous and transgenic kappa gene expression
Autore:
Shen, HM; Peters, A; Kao, D; Storb, U;
Indirizzi:
Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA Univ ChicagoChicago IL USA 60637 ochem & Mol Biol, Chicago, IL 60637 USA Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 enet & Cell Biol, Chicago, IL 60637 USA
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 5, volume: 13, anno: 2001,
pagine: 665 - 674
SICI:
0953-8178(200105)13:5<665:T3'IKE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; SOMATIC HYPERMUTATION; INTRON ENHANCER; TERMINAL DOMAIN; SPLICE SITES; TRANSCRIPTION; SEQUENCES; REGION; ELEMENTS; POLYADENYLATION;
Keywords:
Ig genes; non-consensus RNA splicing; RNA polymerase II; RNA polymerase III; somatic hypermutation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Storb, U Univ Chicago, Dept Biochem & Mol Biol, 920 E 58th St, Chicago, IL60637 USA Univ Chicago 920 E 58th St Chicago IL USA 60637 ago, IL 60637 USA
Citazione:
H.M. Shen et al., "The 3 ' Ig kappa enhancer contains RNA polymerase II promoters: implications for endogenous and transgenic kappa gene expression", INT IMMUNOL, 13(5), 2001, pp. 665-674

Abstract

We have created a Ic transgene in which a polymerase (pol) III promoter replaces the pol II promoter. Two independent transgenic lines show somatic hypermutation of the transgene in B cells from hyperimmunized mice. Both lines transcribe transgenes from the pol III promoter in the liver. However, in spleen and spleen B cell-derived hybridomas, they also transcribe mRNA from pol II promoters located within the 3' kappa enhancer of the preceding transgene copy in a tandem transgene array. The findings demonstrate that inan array of multiple transgenes the expression (and somatic hypermutation)of an individual transgene copy must be considered in the context of the other copies. We also show that sequences around the 3' kappa enhancer in endogenous genes are transcribed. The possible role of these promoters in endogenous kappa gene expression is discussed. An unrelated finding in this study was a novel RNA splice in one hybridoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 08:45:09