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Titolo:
Differential expression of sympathoadrenal lineage-determining genes and phenotypic markers in cultured primary neural crest cells
Autore:
Bilodeau, ML; Boulineau, T; Greulich, JDM; Hullinger, RL; Andrisani, OM;
Indirizzi:
Purdue Univ, Dept Basic Med Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 c Med Sci, W Lafayette, IN 47907 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 3, volume: 37, anno: 2001,
pagine: 185 - 192
SICI:
1071-2690(200103)37:3<185:DEOSLG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTRANSMITTER BIOSYNTHETIC GENES; DEVELOPING SYMPATHETIC NEURONS; ACHAETE-SCUTE HOMOLOG-1; TYROSINE-HYDROXYLASE; HOMEODOMAIN PROTEINS; ADRENERGIC CELLS; CYCLIC-AMP; TRANSCRIPTION; PHOX2A; MASH1;
Keywords:
cytokines; growth factors; adhesion factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Andrisani, OM Purdue Univ, Dept Basic Med Sci, 1246 Lynn Hall, W Lafayette, IN 47907 USA Purdue Univ 1246 Lynn Hall W Lafayette IN USA 47907 7907 USA
Citazione:
M.L. Bilodeau et al., "Differential expression of sympathoadrenal lineage-determining genes and phenotypic markers in cultured primary neural crest cells", IN VITRO-AN, 37(3), 2001, pp. 185-192

Abstract

Bone morphogenetic protein-2 (BMP-2) promotes the development of primary neural crest cells grown in tissue culture to the sympathoadrenal (SA) lineage. Independent studies have characterized the expression patterns of SA-lineage genes in developing chicken embryo; however, studies using cultured primary neural crest cells have characterized only the expression patterns of the catecholaminergic markers, tyrosine hydroxylase (TH) and catecholamines (CAs). To further explore the molecular mechanisms that control SA-cell development using the in vitro model system, it is crucial to define the expression patterns of both the catecholaminergic markers and the genes: regulating SA-lineage determination. Accordingly, we defined, in the absence and presence of BMP-2, the temporal expression patterns of TH and CA, the SA lineage-determining genes ASH-1, Phox2a, and Phox2b, the GATA-2 gene, and the pan-neuronal SCG10 gene. Comparison of these data with the reported temporal and spatial patterns of expression in vivo demonstrate that the inductive steps of SA-lineage determination, including the specification of neurotransmitter identity and neuronal fate, are recapitulated in the neural-crest culture system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 13:34:20