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Titolo:
Permanent phenotypic and genotypic changes of prostate cancer cells cultured in a three-dimensional rotating-wall vessel
Autore:
Rhee, HW; Zhau, HE; Pathak, S; Multani, AS; Pennanen, S; Visakorpi, T; Chung, LWK;
Indirizzi:
Univ Virginia, Sch Med, Dept Urol, Mol Urol & Therapeut Program, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Texas, MD Anderson Canc Ctr, Dept Biol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 anc Ctr, Dept Biol, Houston, TX 77030 USA Univ Tampere, Dept Canc Genet, Tampere 33100, Finland Univ Tampere Tampere Finland 33100 pt Canc Genet, Tampere 33100, Finland
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 3, volume: 37, anno: 2001,
pagine: 127 - 140
SICI:
1071-2690(200103)37:3<127:PPAGCO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; SIMULATED MICROGRAVITY; 3-DIMENSIONAL CULTURES; BONE FIBROBLASTS; CARCINOMA-CELLS; GROWTH-INVIVO; TUMOR; ESTABLISHMENT; PROGRESSION; METASTASIS;
Keywords:
prostate cancer cells in 3D culture; chromosomal losses and gains; anchorage-dependent and -independent growth stromal-epithelial interactions; prostate cancer-bone stroma interactions; microgravity-simulated cell culture; prostate cancer metastasis; prostate cancel models; EGF; bFGF; KGF; HGF/SF; IGF-1 growth factors; responsiveness to growth factors; responsiveness to androgens and estrogens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, LWK Univ Virginia, Sch Med, Dept Urol, Mol Urol & Therapeut Program, POB 800422, Charlottesville, VA 22908 USA Univ Virginia POB 800422 Charlottesville VA USA 22908 22908 USA
Citazione:
H.W. Rhee et al., "Permanent phenotypic and genotypic changes of prostate cancer cells cultured in a three-dimensional rotating-wall vessel", IN VITRO-AN, 37(3), 2001, pp. 127-140

Abstract

A three-dimensional (3D) integrated rotating-wall vessel cell-culture sr;stem was used to evaluate the interaction between a human prostate cancer cell line, LNCaP, and microcarrier heads alone, or microcarrier beads previously seeded with either prostate or bone stromal cells. Upon coculture of LNCaP cells with microcarrier beads either in the presence or in the absence of prostate or bone stromal cells, 3D prostate organoids were formed with the expected hormonal responsiveness to androgen, increased cell growth, anti prostate-specific antigen production. in this communication, we define permanent phenotypic and genotypic changes of LNCaP cells upon coculture withmicrocarrier beads alone, or with microcarrier beads previously seeded with either prostate or bone stromal cells, Most notably, rye observed selective genetic changes, i.e., chromosomal losses or gains, as evaluated by bothconventional cytogenetic and comparative genomic hybridization, in LNCaP sublines derived from the prostate organoids. Moreover, the derivative LNCaPcells appear to hare altered growth profiles, and exhibit permanent and stable changes in response to androgen, estrogen, and growth factors. The derivative LNCaP sublines showed increased anchorage-independent growth rate, and enhanced tumorigenicity and metastatic potential when inoculated orthotopically in castrated athymic mice. Our results support the hypothesis thatfurther nonrandom genetic and phenotypic changes in prostate cancer epithelial cells can occur through an event that resembles "adaptive mutation" such as has been described in bacteria subjected to nutritional starvation. The occurrence of such permanent changes may be highly contact dependent, and appears to be driven by specific micro environmental factors surrounding the tumor cell epithelium grown as 3D prostate organoids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:53:15