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Titolo:
Role of DNA ligase in the illegitimate recombination that generates lambdabio-transducing phages in Escherichia coli
Autore:
Onda, M; Yamaguchi, J; Hanada, K; Asami, Y; Ikeda, H;
Indirizzi:
Kitasato Inst, Ctr Basic Res, Minato Ku, Tokyo 1088642, Japan Kitasato Inst Tokyo Japan 1088642 c Res, Minato Ku, Tokyo 1088642, Japan Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Tokyo, Inst Med Sci, Tokyo 1088639, Japan Univ Tokyo, Biotechnol Res Ctr, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Biotechnol Res Ctr, Tokyo 1138657, Japan Univ Tokyo, Dept Appl Biol Chem, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Dept Appl Biol Chem, Tokyo 1138657, Japan
Titolo Testata:
GENETICS
fascicolo: 1, volume: 158, anno: 2001,
pagine: 29 - 39
SICI:
0016-6731(200105)158:1<29:RODLIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND BREAK REPAIR; NUCLEOTIDE-SEQUENCE; JUNCTIONS; HOTSPOT; GENE; IV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, H Kitasato Inst, Ctr Basic Res, Minato Ku, 5-9-1 Shirokane, Tokyo 1088642, Japan Kitasato Inst 5-9-1 Shirokane Tokyo Japan 1088642 1088642, Japan
Citazione:
M. Onda et al., "Role of DNA ligase in the illegitimate recombination that generates lambdabio-transducing phages in Escherichia coli", GENETICS, 158(1), 2001, pp. 29-39

Abstract

We studied the role of DNA ligase in illegitimate recombination in Escherichia coli. A temperature-sensitive mutation in the lig gene reduced the frequency with which lambda bio-transducing phages were generated to 10-14% ofthat of wild type under W irradiation. Reintroduction of the lig gene intothis mutant restored tire frequency of recombinant phage generation to that of wild type. Furthermore, overexpression of DNA ligase enhanced illegitimate recombination by 10-fold with or without W irradiation. In addition, when DNA ligase was present in only limited amounts, UV-induced or spontaneous illegitimate recombination occurred exclusively at hotspot sites that have relatively long sequences of homology (9 or 13 bp). However, when DNA ligase was overexpressed, most of the illegitimate recombination took place at non-hotspot sites having only short sequences of homology (<4 bp). Thus, the level of ligase activity affects the frequency of illegitimate recombination, the length of sequence homology at the recombination sites, and the preference for recombination at hotspots, at least after W irradiation. These observations support our hypothesis that the illegitimate: recombinationthat generates lambda bio-transducing phages is mediated bp tire DNA break-and-join mechanism.

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Documento generato il 04/04/20 alle ore 09:05:14