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Titolo:
Induction of IRF-3/-7 kinase and NF-kappa B in response to double-strandedRNA and virus infection: common and unique pathways
Autore:
Iwamura, T; Yoneyama, M; Yamaguchi, K; Suhara, W; Mori, W; Shiota, K; Okabe, Y; Namiki, H; Fujita, T;
Indirizzi:
Tokyo Metropolitan Inst Med Sci, Dept Tumor Cell Biol, Bunkyo Ku, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan Waseda Univ, Sch Educ, Dept Biol, Shinjuku Ku, Tokyo 1690051, Japan WasedaUniv Tokyo Japan 1690051 Biol, Shinjuku Ku, Tokyo 1690051, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 4, volume: 6, anno: 2001,
pagine: 375 - 388
SICI:
1356-9597(200104)6:4<375:IOIKAN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFERON REGULATORY FACTOR-3; DEPENDENT PROTEIN-KINASE; TRANSCRIPTION FACTOR COMPLEX; IFN-BETA-GENE; POSITIVE FEEDBACK; ACTIVATION; EXPRESSION; PKR; INVOLVEMENT; DISTINCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, T Tokyo Metropolitan Inst Med Sci, Dept Tumor Cell Biol, Bunkyo Ku, 3-18-22 Honkomagome, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci3-18-22 Honkomagome Tokyo Japan 1138613
Citazione:
T. Iwamura et al., "Induction of IRF-3/-7 kinase and NF-kappa B in response to double-strandedRNA and virus infection: common and unique pathways", GENES CELLS, 6(4), 2001, pp. 375-388

Abstract

Background: Infection by virus or treatment with double-stranded RNA (dsRNA) results in the activation of transcription factors including IRF-3, IRF-7 and a pleiotropic regulator NF-kappaB by specific phosphorylation. These factors are important in triggering a cascade of antiviral responses. A protein kinase that is yet to be identified is responsible for the activation of these factors and plays a key role in the responses. Results: The signal cascade was analysed using sensitive assays for the activation of IRF-3 and NF-kappaB, and various inhibitors. We found that the activation of IRF-3 and NF-kappaB by dsRNA or virus involves a process thatis sensitive to Geldanamycin. Although the induction of NF-kappaB by dsRNA/virus and TNF-alpha involves common downstream pathways including IKK activation, the upstream, Geldanamycin-sensitive process was unique to the dsRNA/virus-induced signal. By an in vitro assay using cell extract, we found an inducible protein kinase activity with physiological specificity of IRF-3phosphorylation. Furthermore, the same extract specifically phosphorylatedIRF-7 in a similar manner. Conclusions: Double-stranded RNA or virus triggers a specific signal cascade that results in the activation of the IRF-3/-7 kinase we detected, whichcorresponds to the long-sought signalling machinery that is responsible for triggering the early phase of innate response. The signal branches to a common NF-kappaB activation cascade, thus resulting in the activation of a set of critical transcription factors for the response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:47:02