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Titolo:
Defective development of secretory neurones in the hypothalamus of Arnt2-knockout mice
Autore:
Hosoya, T; Oda, Y; Takahashi, S; Morita, M; Kawauchi, S; Ema, M; Yamamoto, M; Fujii-Kuriyama, Y;
Indirizzi:
Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan TohokuUniv Sendai Miyagi Japan 9808578 em, Sendai, Miyagi 9808578, Japan Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 sukuba, Ibaraki 3058577, Japan Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058577, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058577 sukuba, Ibaraki 3058577, Japan Japan Sci & Technol, Core Res Evolut Sci & Technol, Tokyo, Japan Japan Sci& Technol Tokyo Japan Res Evolut Sci & Technol, Tokyo, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 4, volume: 6, anno: 2001,
pagine: 361 - 374
SICI:
1356-9597(200104)6:4<361:DDOSNI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARYL-HYDROCARBON RECEPTOR; SINGLE-MINDED GENE; NUCLEAR TRANSLOCATOR ARNT; CLOSE SEQUENCE SIMILARITY; INDUCIBLE FACTOR 1-ALPHA; CNS MIDLINE DEVELOPMENT; AH DIOXIN RECEPTOR; BHLH-PAS PROTEIN; DEVELOPING MOUSE; CDNA CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Fujii-Kuriyama, Y Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 9808578, Japan
Citazione:
T. Hosoya et al., "Defective development of secretory neurones in the hypothalamus of Arnt2-knockout mice", GENES CELLS, 6(4), 2001, pp. 361-374

Abstract

Background: Within the basic region-helix-loop-helix (bHLH)-PAS family of transcription factors, Arnt and Arnt2 play unique roles; these two factors not only heterodimerize with themselves, but also with other members of this family and they act as transcription regulators which bind to specific DNA elements. Whereas Arnt is broadly expressed in various tissues, the expression of Arnt2 is known to be limited to the neural tissues. Results: To elucidate the function of Arnt2 in detail, we cloned the mouseArnt2 gene and its gene structure was determined. We subsequently generated germ line Arnt2 mutant mice by gene targeting technology. Heterozygous Arnt2 mice were viable, but homozygous Arnt2 gene knockout mice died shortly after birth. Histological and immunological analyses revealed that the supraoptic nuclei (SON) and the paraventricular nuclei (PVN) are hypocellular. Moreover, secretory neurones identified by the expression of neurosecretoryhormone such as arginine vasopressin, oxytocin, corticotrophin-releasing hormone and somatostatin are completely absent in SON and PVN in the mutant Arnt2 mice. Consistent with these observations, prospective SON and PVN neurones which express Brn2 appeared around E13.5 in the mantle zone, but no neurones which expressed the neurosecretory hormones were found in the SON and PVN regions. Conclusions: These data show that the transcription factor Arnt2 controls the development of the secretory neurones at the later or final stages of differentiation rather than at the beginning stage. Strikingly similar observations have been reported with the Sim1 deficient mice. Taken together, our results demonstrate that Arnt2 is an indispensable transcription factor for the development of the hypothalamus, and suggest that Arnt2 is an obligatory partner molecule of Sim1 in the developmental process of the neuroendocrinological cell lineages.

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Documento generato il 01/10/20 alle ore 16:16:15