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Titolo:
Stress-induced apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining
Autore:
Yabu, T; Todoriki, S; Yamashita, M;
Indirizzi:
Natl Res Inst Fisheries Sci, Kanagawa 2368648, Japan Natl Res Inst Fisheries Sci Kanagawa Japan 2368648 nagawa 2368648, Japan Tokyo Univ Fisheries, Tokyo 1088477, Japan Tokyo Univ Fisheries Tokyo Japan 1088477 Fisheries, Tokyo 1088477, Japan Natl Food Res Inst, Tukuba, Ibaraki 3058642, Japan Natl Food Res Inst Tukuba Ibaraki Japan 3058642 a, Ibaraki 3058642, Japan
Titolo Testata:
FISHERIES SCIENCE
fascicolo: 2, volume: 67, anno: 2001,
pagine: 333 - 340
SICI:
0919-9268(200104)67:2<333:SABHSU>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-DEATH; ICE/CED-3 PROTEASE; IDENTIFICATION; GASTRULATION; ENDONUCLEASE; EXPRESSION; MECHANISM; INDUCTION; FAMILY;
Keywords:
apoptosis; caspase; Danio rerio; embryo; heat shook; UV; zebrafish; gamma-ray irradiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Yamashita, M Natl Res Inst Fisheries Sci, Kanagawa 2368648, Japan Natl ResInst Fisheries Sci Kanagawa Japan 2368648 8, Japan
Citazione:
T. Yabu et al., "Stress-induced apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining", FISHERIES S, 67(2), 2001, pp. 333-340

Abstract

Environmental stress-induced apoptosis in zebrafish Danio rerio embryos was characterized by assaying caspase-3-like activity and whole-mount terminal deoxynucleotidyl nick-end labeling (TUNEL) staining. Severe stress conditions, such as heat shock at 39 degreesC for 1 h, ultraviolet light at 10-100 mJ/cm(2) and gamma -ray irradiation at 5-20 Gy induced extensive apoptosis in embryos. Apoptotic cells were observed after the bud and 1-somite stages in normal embryos by TUNEL staining, and after stress treatment many TUNEL-positive cells were found in the enveloping and deep cell layers and thelarval fin. The caspase-3-like activity increased severalfold during stress-induced apoptosis in a dose-dependent manner. These findings indicate that apoptotic pathways, mediated by caspase-3-like activity, play a major role in zebrafish embryogenesis under stress conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:01:05