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Titolo:
Cloning and expression of a putative common cytokine receptor gamma chain (gamma C) gene in rainbow trout (Oncorhynchus mykiss)
Autore:
Wang, TH; Secombes, CJ;
Indirizzi:
Univ Aberdeen, Dept Zool, Aberdeen AB24 2TZ, Scotland Univ Aberdeen Aberdeen Scotland AB24 2TZ ol, Aberdeen AB24 2TZ, Scotland
Titolo Testata:
FISH & SHELLFISH IMMUNOLOGY
fascicolo: 3, volume: 11, anno: 2001,
pagine: 233 - 244
SICI:
1050-4648(200104)11:3<233:CAEOAP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; INTERLEUKIN-2 RECEPTOR; IL-2 RECEPTOR; MOLECULAR-CLONING; HUMAN MONOCYTE; T-CELLS; SEQUENCE; EVOLUTION; CHEMOKINE; IDENTIFICATION;
Keywords:
common cytokine receptor gamma chain; rainbow trout; cDNA; expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Secombes, CJ Univ Aberdeen, Dept Zool, Tillydrone Ave, Aberdeen AB24 2TZ, Scotland Univ Aberdeen Tillydrone Ave Aberdeen Scotland AB24 2TZ land
Citazione:
T.H. Wang e C.J. Secombes, "Cloning and expression of a putative common cytokine receptor gamma chain (gamma C) gene in rainbow trout (Oncorhynchus mykiss)", FISH SHELLF, 11(3), 2001, pp. 233-244

Abstract

A full length cDNA of a putative common cytokine receptor gamma chain (gammaC) gene of rainbow trout (Oncorhynchus mykiss) has been cloned and sequenced. The contiguous cDNA contained 2291 nucleotides, consisting of an ORF of 1029 bp, with a 72 bp 5' UTR and a 1190 bp 3' UTR. The coding region showed 44-46% identity to mammalian gammaC genes. The ORF translated into a 343amino acid protein, with some 28-30% amino acid identity to the coding region of mammalian sequences. A predicted signal peptide and transmembrane domain were identified, giving a 206 amino acid extracellular domain and a 98amino acid intracellular domain in the trout molecule. Five potential glycosylation sites were present in the extracellular domain, as were six conserved cysteine residues and the W-S-X-W-S motif typical of haemopoietin receptors. One of the most interesting differences between the trout and mammalian sequences was the lack of tyrosines in the trout intracellular domain. RT-PCR studies revealed a wide tissue distribution of gammaC expression, with detectable transcript in blood, spleen, gill, kidney, brain and liver. Low levels of gammaC transcript were detectable in unstimulated macrophage cultures and expression was increased by stimulation of the cells with recombinant trout interleukin-1 beta (IL-1 beta) or LPS. Similarly, in the RTG cell line which exhibited even lower level constitutive expression, stimulation with IL-1 beta increased gammaC transcript levels but LPS had no effect. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:02:03