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Titolo:
Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans
Autore:
Moller, P; Loft, S; Lundby, C; Olsen, NV;
Indirizzi:
Univ Copenhagen, Panum Inst, Dept Pharmacol, Inst Publ Hlth, DK-2200 Copenhagen N, Denmark Univ Copenhagen Copenhagen Denmark N Hlth, DK-2200 Copenhagen N, Denmark Copenhagen Univ Hosp, Neurosci Ctr, Dept Neuroanaesthesia, DK-2200 Copenhagen, Denmark Copenhagen Univ Hosp Copenhagen Denmark DK-2200 2200 Copenhagen, Denmark
Titolo Testata:
FASEB JOURNAL
fascicolo: 7, volume: 15, anno: 2001,
pagine: 1181 - 1186
SICI:
0892-6638(200105)15:7<1181:AHAHEI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; INTERLEUKIN-1 RECEPTOR ANTAGONIST; ALTITUDE PULMONARY-EDEMA; HUMAN ENDOTHELIAL-CELLS; MODERATE-ALTITUDE; ALVEOLAR MACROPHAGES; HYPOBARIC HYPOXIA; GENE-EXPRESSION; SKELETAL-MUSCLE; STRESS;
Keywords:
FPG; high-intensity exercise; ENDO III; reactive oxygen species;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Moller, P Univ Copenhagen, Panum Inst, Dept Pharmacol, Inst Publ Hlth, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark Univ Copenhagen Blegdamsvej 3 Copenhagen Denmark N n N, Denmark
Citazione:
P. Moller et al., "Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans", FASEB J, 15(7), 2001, pp. 1181-1186

Abstract

The present study investigated the effect of a single bout of exhaustive exercise on the generation of DNA strand breaks and oxidative DNA damage under normal conditions and at high-altitude hypoxia (4559 meters for 3 days). Twelve healthy subjects performed a maximal bicycle exercise test; lymphocytes were isolated for analysis of DNA strand breaks and oxidatively altered nucleotides, detected by endonuclease III and formamidipyridine glycosylase (FPG) enzymes. Urine was collected for 24 h periods for analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a marker of oxidative DNA damage. Urinary excretion of 8-oxodG increased during the first day in altitude hypoxia, and there were more endonuclease III-sensitive sites on day 3 at high altitude. The subjects had more DNA strand breaks in altitude hypoxia than at sea level. The level of DNA strand breaks further increased immediately after exercise in altitude hypoxia, Exercise-induced generation of DNA strand breaks was not seen at sea level. In both environments, the level of FPG and endonuclease III-sensitive sites remained unchanged immediately after exercise. DNA strand breaks and oxidative DNA damage are probably produced by reactive oxygen species, generated by leakage of the mitochondrial respiration or during a hypoxia-induced inflammation, Furthermore, the presence of DNA strand breaks may play an important role in maintaining hypoxia-induced inflammation processes. Hypoxia seems to deplete the antioxidant system of its capacity to withstand oxidative stress produced by exhaustive exercise.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 08:32:05