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Titolo:
Effects of nitric oxide on normal and ischemic cochlea of the guinea pig
Autore:
Ruan, RS; Leong, SK; Yeoh, KH;
Indirizzi:
Natl Univ Singapore Hosp, Dept Otolaryngol, Singapore 119074, Singapore Natl Univ Singapore Hosp Singapore Singapore 119074 re 119074, Singapore Natl Univ Singapore, Dept Anat, Singapore 119260, Singapore Natl Univ Singapore Singapore Singapore 119260 ngapore 119260, Singapore
Titolo Testata:
EXPERIMENTAL NEUROLOGY
fascicolo: 1, volume: 169, anno: 2001,
pagine: 200 - 207
SICI:
0014-4886(200105)169:1<200:EONOON>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-NITROPRUSSIDE; NEURONAL DEATH; SPINAL-CORD; SYNTHASE-I; GLUTAMATE; MECHANISM; OTOTOXICITY; EXPRESSION; RAT; LOCALIZATION;
Keywords:
guinea pig; cochlea; ischemia; S-nitrosocysteine; nitroglycerin; peroxynitrite; SIN-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ruan, RS Natl Univ Singapore Hosp, Dept Otolaryngol, Lower Kent Ridge Rd, Singapore119074, Singapore Natl Univ Singapore Hosp Lower Kent Ridge Rd Singapore Singapore 119074
Citazione:
R.S. Ruan et al., "Effects of nitric oxide on normal and ischemic cochlea of the guinea pig", EXP NEUROL, 169(1), 2001, pp. 200-207

Abstract

To determine whether nitric oxide (NO)/peroxynitrite plays any role in neurodestruction observed in ischemic cochlea of the guinea pig, the effects of NO donors like S-nitrosocysteine (S-NC) and nitroglycerin (NTG), peroxynitrite generators like 3-morpholinosydnonimine (SIN-1), peroxynitrite inhibitors like superoxide dismutase plus catalase (SOD/Cat), as well as NOS inhibitors like N-G-nitro-L-arginine methyl ether (L-NAME), were tested on normal and ischemic cochleae. Various concentrations of S-NC and SIN-1 were introduced into the perilymphatic space of normal guinea pig cochlea, Quantitative scanning electron microscopy of inner and outer hair cells was carriedout 2 days later. To determine the level of NO in the cochlea after 20 to 120 min of ischemia, nitrites/nitrates in the perilymph were measured. The effects of NO on the ischemic cochlea were tested by infusion of SOD/Cat, L-NAME, S-NC, and NTG; into the perilymphatic space just before decapitation. Introduction of fixative into the cochlea was delayed for 15 min to investigate the effects of the chemicals on nerve endings at the base of inner hair cells. The results showed that the level of nitrites/nitrates tended todecline with increasing time of ischemia. There was no significant hair cell loss in the cochleae treated with SIN-1 or S-NC. At 15 min after ischemia, most of the nerve endings at the base of the inner hair cells were protected from damage when 1 mM S-NC or NTG was infused into the perilymph, Taken together, the results indicate that NO/peroxynitrite is unlikely to be involved in the neurodestruction in the ischemic cochlea. In fact, exogenous NO may have a neural protective effect. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:48:51