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Titolo:
Activated macrophages migrate to the subcutaneous tumor site via the peritoneum: A novel route of cell trafficking
Autore:
Bhaumik, S; Mitra, R; Varalakshmi, C; Khar, A;
Indirizzi:
Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India Ctr Cellular & Mol Biol Hyderabad Andhra Pradesh India 500007 desh, India
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 266, anno: 2001,
pagine: 44 - 52
SICI:
0014-4827(20010515)266:1<44:AMMTTS>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; SPONTANEOUS REGRESSION; RAT HISTIOCYTOMA; NECROSIS-FACTOR; AK-5 TUMOR; T-CELL; CANCER; MECHANISMS; REJECTION; LYMPHOCYTES;
Keywords:
cell trafficking; macrophage activation; free radicals; tumor regression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Khar, A Ctr Cellular & Mol Biol, Uppal Rd, Hyderabad 500007, Andhra Pradesh, India Ctr Cellular & Mol Biol Uppal Rd Hyderabad Andhra Pradesh India 500007
Citazione:
S. Bhaumik et al., "Activated macrophages migrate to the subcutaneous tumor site via the peritoneum: A novel route of cell trafficking", EXP CELL RE, 266(1), 2001, pp. 44-52

Abstract

Spontaneous regression of AK-5 tumor in syngeneic hosts reported earlier involves the interplay of Th1-type cytokines and cell-mediated immunity. Upon subcutaneous transplantation of AK-5 cells, there was accumulation of immune cells in the peritoneum, of which macrophages were the predominant typeand were found to be in a hyperactive state. They released macrophage-derived tumoricidal mediators like NO, O-2(-), and ONOO- which exhibited potentcytotoxic activity against AK-5 cells in vitro. Interestingly, there was adramatic disappearance of these hyperactive cells from the peritoneal cavity which correlated well with the onset of tumor regression at the subcutaneous site. Direct labeling of these cells in the peritoneum by the trackingdye PKH26 showed their migration to the tumor site. Similarly, frozen tumor sections when scanned under confocal microscope clearly exhibited fluorescent macrophages embedded into the tumor. Immunohistochemical sections of these intratumoral macrophages showed nitrotyrosine residues, indicating their contribution in the free-radical-mediated AK-5 cell death, thereby leading to successful tumor remission. These observations suggest a directional migration of the hyperactivated peritoneal population to the tumor site. Wehave also confirmed the influx of macrophages and other immune cells into the peritoneum after sc transplantation of Meth A tumor cells in Balb/c mice. Our studies suggest a role for the peritoneal compartment in imparting appropriate stimulus to the immune cells prior to their participation in theantitumor immune response. These studies suggest a novel route of macrophage trafficking via the peritoneum. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:45:18