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Titolo:
Para-nitropbenol glucuronidation and sulfation in rat and human liver slices
Autore:
Kuhn, UD; Rost, M; Muller, D;
Indirizzi:
Univ Jena, Fac Med, Inst Pharmacol & Toxicol, D-07740 Jena, Germany Univ Jena Jena Germany D-07740 harmacol & Toxicol, D-07740 Jena, Germany
Titolo Testata:
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
fascicolo: 1, volume: 53, anno: 2001,
pagine: 81 - 87
SICI:
0940-2993(200104)53:1<81:PGASIR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
UDP-GLUCURONOSYLTRANSFERASE ACTIVITIES; DRUG-METABOLIZING-ENZYMES; PHENOL SULFOTRANSFERASE; P-NITROPHENOL; BETA-NAPHTHOFLAVONE; 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE; LIQUID-CHROMATOGRAPHY; ISOLATED HEPATOCYTES; PARTIAL-PURIFICATION; FLUOROMETRIC ASSAY;
Keywords:
beta-naphthoflavone; conjugation; drug metabolism; enzyme induction; glucuronidation; in vitro testing; liver slices; para-nitrophenol; phenobarbital; sulfation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kuhn, UD Univ Jena, Fac Med, Inst Clin Pharmacol, D-07740 Jena, Germany Univ Jena Jena Germany D-07740 Pharmacol, D-07740 Jena, Germany
Citazione:
U.D. Kuhn et al., "Para-nitropbenol glucuronidation and sulfation in rat and human liver slices", EXP TOX PAT, 53(1), 2001, pp. 81-87

Abstract

Para-nitrophenol (PNP) is a well-known substrate for both phase I (hydroxylation at cytochrome P450) and phase II reactions (glucuronidation and sulfation). HPLC separation of PNP conjugates has already been described, but not for respective studies with liver slices, which nowadays have proven to be a suitable model for metabolic studies. Therefore we adapted an HPLC method for the simultaneous measurement of PNP glucuronidation (PNP-G) and sulfation (PNP-S) in this in vitro system. Both activities are substantially maintained over an incubation period of 24 h. PNP-G activity, however, seemsto be better preserved, as indicated by stable values for PNP-G but reduced PNP-S values after 48 h liver slice prc incubation. 24 h exposure of the slices to beta-naphthoflavone or phenobarbital does not change PNP-G or PNP-S activities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:20:07