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Titolo:
Stimulatory and synergistic effects of luteinising hormone and insulin like growth factor 1 on the secretion of vascular endothelial growth factor and progesterone of cultured bovine granulosa cells
Autore:
Schams, D; Kosmann, M; Berisha, B; Amselgruber, WM; Miyamoto, A;
Indirizzi:
Tech Univ Munich, Inst Physiol, D-85350 Freising, Germany Tech Univ Munich Freising Germany D-85350 iol, D-85350 Freising, Germany Univ Hohenheim, Dept Anat & Physiol, D-7000 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-7000 ysiol, D-7000 Stuttgart, Germany
Titolo Testata:
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES
fascicolo: 3, volume: 109, anno: 2001,
pagine: 155 - 162
SICI:
0947-7349(2001)109:3<155:SASEOL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; PROTEIN-KINASE-C; CORPUS-LUTEUM; ESTROUS-CYCLE; TISSUE CONCENTRATION; GENE-EXPRESSION; IN-VITRO; ANGIOGENESIS; RECEPTORS; PREGNANCY;
Keywords:
granulosa cells; vascular endothelial growth factor; LH; IGF1; cow;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Schams, D Tech Univ Munich, Inst Physiol, Weihenstephaner Berg 3, D-85350 Freising, Germany Tech Univ Munich Weihenstephaner Berg 3 Freising GermanyD-85350
Citazione:
D. Schams et al., "Stimulatory and synergistic effects of luteinising hormone and insulin like growth factor 1 on the secretion of vascular endothelial growth factor and progesterone of cultured bovine granulosa cells", EXP CL E D, 109(3), 2001, pp. 155-162

Abstract

Vascular endothelial growth factor (VEGF) is the most important factor in the regulation of angiogenesis. Associated with luteinisation and formationof corpus luteum (CL) are alterations in luteal vascularity. The aim of the study was to test under in vitro conditions' the stimulation of VEGF and progesterone (P) secretion of bovine granulosa cells by LH, IGF1 (insulin like growth factor) or by factors known to be produced by luteinised granulosa cells or in the early CL. Localisation of VEGF protein in preovulatory follicle and early CL were achieved by immunohistochemistry. LH and IGF1 stimulated dose dependently and significantly P and VEGF when tested alone. Both hormones added simultaneously had clear additive and even more interesting far greater (synergistic) effects on P with LH (0.1 ng/ml) plus 5 or 10 ng IGF1. In contrast, VEGF was stimulated only additively with 0.1 ng/ml ofLH plus 5 or 10 ng IGF1. But with the higher dose of LH (1 ng/ml) additionally to the additive effect a tendency for a synergistic action (which was significant with 1 ng LH plus 5 ng IGF1/ml) was observed. Endothelin, oxytocin, progesterone, atrial natiuretic peptide, angiotensin II, prostaglandinF2 alpha alpha, prostaglandin-E-2, cortisol, fibroblast growth factor 1 and 2 and growth hormone showed no effect neither on P nor on VEGF. Tumour necrosis factor alpha (TNF alpha) stimulated (P<0.05) VEGF with 10 or 100 ng/ml but not P. TPA (12-0 tetra decaenoyl-phorbol-13-acetate) or Ca2+ ionophore did not show a stimulatory effect in contrast to forskolin which increased P and VEGF secretion dose dependently. The VEGF protein was localised infollicle (granulosa cells, theca cells and some endothelial cells) and early (about 24 h after ovulation) CL (granulosa-lutein cells and endothelial cells). The same signalling pathway by stimulation of cAMP production and proteinkinase A activation for luteinisation and neo-vascularisation demonstrates a close temporal and spatial relationship of these normal physiological processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:19:06