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Titolo:
Expression of Bcl-2 family reduces apoptotic hepatocytes after excessive hepatectomy
Autore:
Kamimukai, N; Togo, S; Hasegawa, S; Kubota, T; Kurosawa, H; Li, XK; Suzuki, S; Shimada, H;
Indirizzi:
Yokohama City Univ, Sch Med, Dept Surg 2, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Natl Childrens Med Res Ctr, Dept Expt Surg & Bioengn, Tokyo 154, Japan Natl Childrens Med Res Ctr Tokyo Japan 154 g & Bioengn, Tokyo 154, Japan
Titolo Testata:
EUROPEAN SURGICAL RESEARCH
fascicolo: 1, volume: 33, anno: 2001,
pagine: 8 - 15
SICI:
0014-312X(2001)33:1<8:EOBFRA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; ANTI-FAS ANTIBODY; CYTOCHROME-C; LIVER-REGENERATION; CELL-DEATH; INDUCTION; RELEASE; GROWTH; GENE; MICE;
Keywords:
liver apoptosis; liver regeneration; Bcl-xL; Bcl-2; gene transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kamimukai, N Yokohama City Univ, Sch Med, Dept Surg 2, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ 3-9 Fukuura Yokohama Kanagawa Japan 2360004
Citazione:
N. Kamimukai et al., "Expression of Bcl-2 family reduces apoptotic hepatocytes after excessive hepatectomy", EUR SURG RE, 33(1), 2001, pp. 8-15

Abstract

Excessive hepatectomy often causes fatal hepatic failure, but the mechanism is unknown. We used a novel protocol of partial 90 and 95% hepatectomy (PHx) to investigate this mechanism in 2 groups of rats. The 90% PHx rats survived, but the 95% PHx animals died of hepatic failure. In the latter, cytokine (interleukin-6, tumor necrosis factor-a) levels and the apoptotic hepatocyte count increased, and there were few mitotic cells. By contrast, in the 90% PHx rats, the mitotic cell count increased, and more anti-apoptotic Bcl-xL protein was expressed. These results demonstrate that expression of Bcl-xL protein as an anti-apoptotic factor or regeneration factor contributes to survival after 90% PHx. Using an adenovirus vector, the human bcl-2 gene (hbcl-2) was therefore transfected to DA rat livers where it was efficiently expressed, and then 95% PHx was performed. Liver damage was decreasedand the apoptotic cell count decreased too, but the rats died. We concluded that transfection of the hbcl-2 gene partly prevents cytotoxicity (apoptosis), but cannot ensure survival. Thus, some other factor is required (e.g., a regeneration stimulator) to maintain life in these models. Copyright (C) 2001 S. Karger AG. Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:05:22