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Titolo:
Decreased amount of mpl and reduced expression of glycoprotein IIb/IIIa and glycoprotein Ib on platelets from patients with refractory anemia: analysis by a non-isotopic quantitative ligand binding assay and immunofluorescence
Autore:
Izumi, M; Takeshita, A; Shinjo, K; Naito, K; Matsui, H; Shibata, K; Ohnishi, K; Kanno, T; Ohno, R;
Indirizzi:
Hamamatsu Univ, Sch Med, Dept Internal Med 3, Div Hematol, Hamamatsu, Shizuoka 4313192, Japan Hamamatsu Univ Hamamatsu Shizuoka Japan 4313192 , Shizuoka 4313192, Japan Hamamatsu Univ, Sch Med, Equipment Ctr, Hamamatsu, Shizuoka 43131, Japan Hamamatsu Univ Hamamatsu Shizuoka Japan 43131 atsu, Shizuoka 43131, Japan Hamamatsu Univ, Sch Med, Clin Labs, Hamamatsu, Shizuoka 43131, Japan Hamamatsu Univ Hamamatsu Shizuoka Japan 43131 atsu, Shizuoka 43131, Japan
Titolo Testata:
EUROPEAN JOURNAL OF HAEMATOLOGY
fascicolo: 4, volume: 66, anno: 2001,
pagine: 245 - 252
SICI:
0902-4441(200104)66:4<245:DAOMAR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT-HUMAN-ERYTHROPOIETIN; SERUM THROMBOPOIETIN LEVELS; STIMULATING FACTOR-RECEPTOR; FLOW-CYTOMETRIC ASSAY; MYELODYSPLASTIC SYNDROMES; HEALTHY-VOLUNTEERS; PRECURSOR CELLS; MEGAKARYOCYTOPOIESIS; DIFFERENTIATION; REGULATOR;
Keywords:
mpl; platelet; CD41; CD42b; refractory anemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Takeshita, A Hamamatsu Univ, Sch Med, Dept Internal Med 3, Div Hematol, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan Hamamatsu Univ 1-20-1 Handayama Hamamatsu Shizuoka Japan 4313192
Citazione:
M. Izumi et al., "Decreased amount of mpl and reduced expression of glycoprotein IIb/IIIa and glycoprotein Ib on platelets from patients with refractory anemia: analysis by a non-isotopic quantitative ligand binding assay and immunofluorescence", EUR J HAEMA, 66(4), 2001, pp. 245-252

Abstract

Using a non-isotopic Ligand binding assay and immunofluorescence, we examined the amount of mpl, glycoprotein IIb/IIIa (gpIIb/IIIa) and glycoprotein Ib (gpIb) on platelets from healthy volunteers and patients with refractoryanemia (RA). For the analysis of mpl expression, we applied both a non-isotopic ligand binding assay and immunofluorescence using anti-mpl monoclonalantibody, and compared the results from both methods. The non-isotopic ligand binding assay has been developed in our laboratory and is suitable for the quantitative analysis of a small amount of cytokine receptors such as mpl on platelets. In platelets from patients with RA, the amount of mpl expressed by the D value was 0.05 +/- 0.03 (mean +/- standard deviation), and was significantly lower than that in healthy volunteers (0.15 +/- 0.05, p < 0.0001). The mean fluorescence intensities (MFI) of gpIIb/IIIa and gpIb on platelets from RA patients were 28.8 +/- 8.8 and 20.8 +/- 7.7, respectively, and were significantly lower than those on normal subjects (93.2 +/- 22.6and 67.4 +/- 9.1, p < 0.0001 and p < 0.0001, respectively). There was a good correlation between the amount of mpl and the MFI of gpIIb/IIIa (p = 0.794, p < 0.0001) or gpIb (p = 0.774, p < 0.0001), and between those of gpIIb/IIIa and gpIb (p = 0.728, p < 0.0001). We demonstrated a decreased amount of mpl as well as a reduced expression of gpIIb/IIIa and gpIb on platelets from RA patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:50:32