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Titolo:
Methods for the determination of binding constants by capillary electrophoresis
Autore:
Rundlett, KL; Armstrong, DW;
Indirizzi:
Iowa State Univ Sci & Technol, Ames Lab, Dept Chem, Ames, IA 50011 USA Iowa State Univ Sci & Technol Ames IA USA 50011 Chem, Ames, IA 50011 USA Pfizer Inc, Groton, CT 06340 USA Pfizer Inc Groton CT USA 06340Pfizer Inc, Groton, CT 06340 USA
Titolo Testata:
ELECTROPHORESIS
fascicolo: 7, volume: 22, anno: 2001,
pagine: 1419 - 1427
SICI:
0173-0835(200104)22:7<1419:MFTDOB>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ALPHA(1)-ACID GLYCOPROTEIN; MONTE-CARLO SIMULATION; DRUG-PROTEIN BINDING; ASSOCIATION CONSTANTS; ZONE-ELECTROPHORESIS; FRONTAL ANALYSIS; BETA-CYCLODEXTRIN; ENANTIOSELECTIVE BINDING; RECTANGULAR HYPERBOLAE; LIQUID-CHROMATOGRAPHY;
Keywords:
molecular association; equilibrium constants; protein binding; pseudophase; cyclodextrins; micelles; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Armstrong, DW Iowa State Univ Sci & Technol, Ames Lab, Dept Chem, Ames, IA50011 USA Iowa State Univ Sci & Technol Ames IA USA 50011 A 50011 USA
Citazione:
K.L. Rundlett e D.W. Armstrong, "Methods for the determination of binding constants by capillary electrophoresis", ELECTROPHOR, 22(7), 2001, pp. 1419-1427

Abstract

Apparent equilibrium constants for molecular association (e.g., association constants, binding constants, dissociation constants, partition coefficients) can be determined with a variety of different capillary electrophoresis (CE) approaches. In many cases, the investigated association behavior is between a smaller molecule or ion (i.e., the solute, drug, or analyte of interest) and a larger entity (e.g., proteins, micelles, polymers, chiral selectors such as cyclodextrins, etc.). Each experimental approach has advantages and disadvantages. Frequently, it is the nature of the system being evaluated that determines the optimal experimental approach. Six different CE-based techniques for evaluating binding constants are reviewed. Examples ofeach method, and recent references on its use are given.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:04:10