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Titolo:
Anion-exchange capillary electrochromatography with indirect UV and directcontactless conductivity detection
Autore:
Hilder, EF; Zemann, AJ; Macka, M; Haddad, PR;
Indirizzi:
Univ Tasmania, Australian Ctr Res Separat Sci, Hobart, Tas, Australia UnivTasmania Hobart Tas Australia s Separat Sci, Hobart, Tas, Australia Leopold Franzens Univ, Inst Analyt Chem & Radiochem, Innsbruck, Austria Leopold Franzens Univ Innsbruck Austria & Radiochem, Innsbruck, Austria
Titolo Testata:
ELECTROPHORESIS
fascicolo: 7, volume: 22, anno: 2001,
pagine: 1273 - 1281
SICI:
0173-0835(200104)22:7<1273:ACEWIU>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
OCTADECYL-SULFONATED-SILICA; RETENTION BEHAVIOR; STATIONARY PHASES; INORGANIC ANIONS; ELECTROPHORESIS; SEPARATION; CHROMATOGRAPHY; COLUMN; BASES;
Keywords:
capillary electrochromatography, contactless conductivity detection; indirect UV detection; anion exchange; polymer stationary phase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Sch Chem, Separat Sci Grp, Hobart, Tas, Australia Univ Tasmania Hobart Tas Australia rp, Hobart, Tas, Australia
Citazione:
E.F. Hilder et al., "Anion-exchange capillary electrochromatography with indirect UV and directcontactless conductivity detection", ELECTROPHOR, 22(7), 2001, pp. 1273-1281

Abstract

Conductivity detection is applied to ion-exchange capillary electrochromatography (IE-CEC) with a packed stationary phase, using a capacitively coupled contactless conductivity detector with detection occurring through the packed bed. Columns were packed with a polymeric latex-agglomerate anion-exchanger (Dionex ASS-SC). A systematic approach was used to determine suitable eluants for IE-CEC separations using simultaneous indirect UV and direct conductivity detection. Salicylate and p-toluenesulfonate were identified as potential eluant competing anions having sufficient eluotropic strength to induce changes in separation selectivity, but salicylate was found to be unsuitable with regard to baseline stability. It was also found for both indirect UV and direct conductivity detection that homogenous column packing was imperative, and monitoring of the baseline could be used to assess the homogeneity of the packed bed. Using a p-toluenesulfonate eluant, the separation of eight common anions was achieved in 2.5 min. Direct conductivity detection was found to be superior to indirect UV detection with regard to both baseline stability and detection sensitivity with detection limits of 4-25 mug/L being obtained. However, the calibration for each anion was not linear over more than one order of magnitude. When using conductivity detection, the concentration of the eluant could be varied over a wider range (2.5-50 mM p-toluenesulfonate) than was the case with indirect UV detection (2.5-10 mM), thereby allowing greater changes in separation selectivity to beachieved. By varying the concentration of p-toluenesulfonate in the eluant, the separation selectivity could be manipulated from being predominantly ion-exchange in nature (2.5 mM) to predominantly electrophoretic in nature (50 mM).

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Documento generato il 07/07/20 alle ore 22:30:32