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Titolo:
Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae)
Autore:
Datta, A; Ghosh, AK; Kundu, SC;
Indirizzi:
Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India Indian Inst Technol Kharagpur W Bengal India 721302 1302, W Bengal, India
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 1, volume: 129, anno: 2001,
pagine: 197 - 204
SICI:
1096-4959(200105)129:1<197:DEOTFG>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMEODOMAIN BINDING-SITES; LIGHT-CHAIN GENE; BOMBYX-MORI; GALLERIA-MELLONELLA; HEAVY-CHAIN; GLAND; PROTEIN; TRANSCRIPTION; PERNYI; RNA;
Keywords:
Antheraea mylitta; fibroin gene; saturniid silkworm; DNA dot blot; moult and intermoult; northern blot; silkgland; silk protein; temporal variations; transcriptional control; western blot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kundu, SC Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India Indian Inst Technol Kharagpur W Bengal India 721302 ngal, India
Citazione:
A. Datta et al., "Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae)", COMP BIOC B, 129(1), 2001, pp. 197-204

Abstract

Fibroin gene expression during the larval developmental stages of the Saturniid silkworm, Antheraea mylitta, was analyzed. Northern blot analysis of larval silk gland total RNA using the fibroin gene as a probe showed that fibroin is expressed in the intermoult stages and repressed during the moulting stages. Abundance of fibroin transcripts gradually increased from the third to fifth intermoult stage. reaching a peak in the fifth intermoult. Transcripts declined during the early spinning stage. Western blot analysis of fibroin protein production with anti-fibroin antibody confirmed the differential fibroin expression, in accordance with fibroin mRNA synthesis. Dot blot hybridization of genomic DNA isolated from each larval developmental stage with the labelled fibroin gene showed that at the genomic level, the relative concentration of the fibroin gene was constant throughout the developmental stages. Our data confirm that fibroin gene expression in A. mylitta. like in B, mori, is transcriptionally controlled and shows differential temporal variations. (C) 2001 Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:55:19