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Titolo:
Role of platelet glycoprotein IIb/IIIa in ADP-activated platelet adhesion to aortic endothelial cells in vitro: observation with video-enhanced contrast microscopy
Autore:
Tomita, Y; Tanahashi, N; Tomita, M; Itoh, Y; Yokoyama, M; Takeda, H; Schiszler, I; Fukuuchi, Y;
Indirizzi:
Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Tokyo, Japan Keio Univ Tokyo Japan , Sch Med, Dept Neurol, Shinjuku Ku, Tokyo, Japan
Titolo Testata:
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
fascicolo: 1, volume: 24, anno: 2001,
pagine: 1 - 9
SICI:
1386-0291(2001)24:1<1:ROPGII>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GPIIB/IIIA ANTAGONIST; GUINEA-PIGS; IN-VITRO; FIBRINOGEN; ADHERENCE; FLOW; SUBENDOTHELIUM; AGGREGATION; INTEGRIN; COLLAGEN;
Keywords:
ADP; platelet; endothelial cell; VEC microscopy; adhesion; GP IIb/IIIa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tomita, Y Keio Univ, Sch Med, Dept Neurol, Shinjuku Ku, Shinanomachi 35, Tokyo, Japan Keio Univ Shinanomachi 35 Tokyo Japan nomachi 35, Tokyo, Japan
Citazione:
Y. Tomita et al., "Role of platelet glycoprotein IIb/IIIa in ADP-activated platelet adhesion to aortic endothelial cells in vitro: observation with video-enhanced contrast microscopy", CL HEMORH M, 24(1), 2001, pp. 1-9

Abstract

Intravascular thrombus formation downstream of cerebral arterial occlusionmay result in necrosis of ischemic tissue. To clarify the causative mechanisms, interaction between adenosine-5'-diphosphate (ADP)-activated platelets and cultured human aortic endothelial cells (HAEC) was examined by employing video enhanced contrast-differential interference contrast (VEC-DIC) microscopy. The numbers of (1) control/platelets, (2) ADP-activated platelets, (3) ADP-activated, anti-platelet GP Iba antibody (GUR20-5)-treated platelets, and (4) ADP-activated, platelet GP IIb/IIIa antagonist (TAK-MB)-treated platelets, associated with HAEC after superfusion and wash-out were counted in visual fields of 30 x 30 mum(2). Many ADP-activated platelets adheredto HAEC directly, while almost no platelets adhered to HAEC in the control. The adhesion was almost completely blocked by the GP IIb/IIIa antagonist,but not by GP lb(x antibody. We conclude that initial binding of ADP-activated platelets to HAEC is mediated by platelet GP IIb/lIIa in this in vitrosystem.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:23:58