Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
New assay for measuring cell surface hydrophobicities of Candida dubliniensis and Candida albicans
Autore:
Jabra-Rizk, MA; Falkler, WA; Merz, WG; Meiller, TF;
Indirizzi:
UMAB, Sch Dent, Dept Oral Med, Baltimore, MD 21201 USA UMAB Baltimore MD USA 21201 Dent, Dept Oral Med, Baltimore, MD 21201 USA UMAB, Sch Dent, Dept OCBS, Baltimore, MD 21201 USA UMAB Baltimore MD USA 21201 Sch Dent, Dept OCBS, Baltimore, MD 21201 USA Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21218 USA Johns Hopkins Univ Baltimore MD USA 21218 Pathol, Baltimore, MD 21218 USA
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 3, volume: 8, anno: 2001,
pagine: 585 - 587
SICI:
1071-412X(200105)8:3<585:NAFMCS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUSOBACTERIUM-NUCLEATUM; EPITHELIAL-CELLS; COAGGREGATION; ADHERENCE; IDENTIFICATION; EXPRESSION; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Jabra-Rizk, MA UMAB, Sch Dent, Dept Oral Med, 666 W Baltimore St, Baltimore, MD 21201 USA UMAB 666 W Baltimore St Baltimore MD USA 21201 MD 21201 USA
Citazione:
M.A. Jabra-Rizk et al., "New assay for measuring cell surface hydrophobicities of Candida dubliniensis and Candida albicans", CL DIAG LAB, 8(3), 2001, pp. 585-587

Abstract

Hydrophobic interactions, based on cell surface hydrophobicity (CSH), are among the many and varied mechanisms of adherence deployed by the pathogenic yeast Candida albicans, Recently it was shown that, unlike C. albicans, C. dubliniensis is a species that exhibits an outer fibrillar layer consistent with constant CSH, Previously, C. dubliniensis grown at 25 or 37 degreesC was shown to coaggregate with the oral anaerobic bacterium Fusobacterium nucleatum, C. albicans, however, demonstrated similar coaggregation only when hydrophobic or grown at 25 degreesC. This observation implied that coaggregation of Candida cells with F. nucleatum is associated with a hydrophobic yeast cell surface. To test this hypothesis, 42 C. albicans and 40 C. dubliniensis clinical isolates, including a C. albicans hydrophobic variant, were grown at 25 and 37 degreesC and tested with the established hydrophobicity microsphere assay, which determines CSH levels based on the number of microspheres attached to the yeast cells. The coaggregation assay was performed in parallel experiments. All C. dubliniensis isolates grown at either temperature, hydrophobic 25 degreesC-grown C. albicans isolates, and the C. albicans hydrophobic variant, unlike the 37 degreesC-hydrophilic C. albicans isolates, exhibited hydrophobic CSH levels with the microsphere assay andsimultaneously showed maximum, 4+, coaggregation with F. nucleatum, The parallel results obtained for C. dubliniensis using both assays support the use of the CoAg assay both as a rapid assay to determine CSH and to differentiate between C. dubliniensis and C. albicans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:16:29