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Titolo:
Detection of heme-binding proteins in epidemic strains of Burkholderia cepacia
Autore:
Smalley, JW; Charalabous, P; Birss, AJ; Hart, CA;
Indirizzi:
Univ Liverpool, Oral Biol Unit, Dept Clin Dent Sci, Liverpool L69 3GN, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3GN , Merseyside, England Univ Liverpool, Dept Med Microbiol & Genito Urinary Med, Liverpool L69 3GN, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3GN , Merseyside, England
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 3, volume: 8, anno: 2001,
pagine: 509 - 514
SICI:
1071-412X(200105)8:3<509:DOHPIE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORPHYROMONAS-GINGIVALIS W50; IRON PROTOPORPHYRIN-IX; PSEUDOMONAS-CEPACIA; CYSTIC-FIBROSIS; SURFACE PROTEIN; OUTER-MEMBRANE; AERUGINOSA; SIDEROPHORE; INFECTION; VIRULENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Smalley, JW Univ Liverpool, Oral Biol Unit, Dept Clin Dent Sci, Edwards Bldg,Daulby St, Liverpool L69 3GN, Merseyside, England Univ Liverpool EdwardsBldg,Daulby St Liverpool Merseyside England L69 3GN
Citazione:
J.W. Smalley et al., "Detection of heme-binding proteins in epidemic strains of Burkholderia cepacia", CL DIAG LAB, 8(3), 2001, pp. 509-514

Abstract

A panel of 30 previously characterized strains representing five genomovars from the Burkholderia cepacia complex (E, Mahenthiralingam, T, Coenye, J. W. Chung, D, P, Speert, J. R, W, Govan, P, Taylor, and P, Vandamme, J, Clin, Microbiol. 38:910-913, 2000) were examined for their iron protoporphyrinIX-binding ability. These included B. cepacia genomovars I and III and B, stabilis (formerly B. cepacia genomovar IV), B, multivorans (formerly B, cepacia genomovar II), and B, vietnamiensis (formerly B, cepacia genomovar V), Cells were exposed to mu -ore bisheme of iron protoporphyrin IX (mu -oxo dimers) and examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing, nondenaturing conditions for the presence of heme-binding proteins using tetramethylbenzidine-H2O2 staining. Seven of the 30strains, each belonging to B. cepacia genomovar III and designated epidemic (in possessing the B, cepacia epidemic strain marker), expressed a 96- to100-kDa heme-binding protein which was located in the outer membrane. The heme-binding protein of B, cepacia genomovar III epidemic strain C5424 bound iron(III) protoporphyrin IX in both the monomeric and mu -oxo bisheme forms. Cells of all strains grown on Columbia agar bound iron protoporphyrin IX in the Ir-oxo bisheme (dimeric) form. There were no statistical differences between the five genomovars, or those possessing the heme-binding protein, in their Cr-oxo bisheme-binding ability. Possession of the outer membrane heme-binding protein may be a pathogenicity trait in enabling the bacterium to withstand oxidative stresses in inflammatory exudates in the lung andmay aid identification of invasive epidemic strains of B, cepacia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:38:25