Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Modulation of endothelial cell morphogenesis in vitro by MMP-9 during glial-endothelial cell interactions
Autore:
Chandrasekar, N; Jasti, S; Alfred-Yung, WK; Ali-Osman, F; Dinh, DH; Olivero, WC; Gujrati, M; Kyritsis, AP; Nicolson, GL; Rao, JS; Mohanam, S;
Indirizzi:
Univ Illinois, Coll Med, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 d, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Pathol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 Med, Dept Pathol, Peoria, IL 61656 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurooncol, Houston, TX USA Univ Texas Houston TX USA son Canc Ctr, Dept Neurooncol, Houston, TX USA Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX USA Univ Texas Houston TX USA rson Canc Ctr, Dept Neurosurg, Houston, TX USA Inst Mol Med, Huntington Beach, CA USA Inst Mol Med Huntington Beach CA USA t Mol Med, Huntington Beach, CA USA
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 4, volume: 18, anno: 2000,
pagine: 337 - 342
SICI:
0262-0898(2000)18:4<337:MOECMI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; MATRIX METALLOPROTEINASES; TISSUE INHIBITOR; IN-VITRO; ANGIOGENESIS INVITRO; PROTEIN-KINASE; INDUCTION; CANCER; DIFFERENTIATION; EXPRESSION;
Keywords:
angiogenesis; extracellular matrix; glioblastoma; MMP-9; TIMPs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Mohanam, S Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, 1 Illini Dr,Box 1649, Peoria, IL 61656 USA Univ Illinois 1 Illini Dr,Box 1649 Peoria IL USA 61656 1656 USA
Citazione:
N. Chandrasekar et al., "Modulation of endothelial cell morphogenesis in vitro by MMP-9 during glial-endothelial cell interactions", CLIN EXP M, 18(4), 2000, pp. 337-342

Abstract

The purpose of this study was to investigate the roles of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the formation of capillary structures by human brain microvascular endothelial cells cocultured with SNB19 glioblastoma cells. Unstimulated coculturesdid not form capillaries and produce MMP-9 but stimulation with the protein kinase C (PKC) activator 4-phorbol-12-myristate 13-acetate (PMA) producedMMP-9 and capillary networks. Addition of recombinant MMP-9 increased capillary formation. Anti-MMP-9 antibodies, TIMP-1, the synthetic MMPs inhibitor Batimastat (BB-94), and the PKC inhibitor calphostin-C all reduced MMP-9 activity and capillary network formation in these cocultures. Cytochalasin-D in the presence of PMA suppressed MMP-9 expression and capillary formation, but colchicine-B had no such effect. Finally, PMA-induced MMP-9 expression and capillary formation were inhibited by the MEKK-specific inhibitor PD98059. These results suggest that MMP-9 is important in endothelial cell morphogenesis and the formation of capillaries in glial/endothelial cocultures in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:20:42