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Titolo:
Role of tissue factor pathway inhibitor-2 (TFPI-2) in amelanotic melanoma (C-32) invasion
Autore:
Konduri, SD; Tasiou, A; Chandrasekar, N; Nicolson, GL; Rao, JS;
Indirizzi:
Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 ci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 d, Dept Neurosurg, Peoria, IL 61656 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Inst Mol Med, Huntington Beach, CA USA Inst Mol Med Huntington Beach CA USA t Mol Med, Huntington Beach, CA USA
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 4, volume: 18, anno: 2000,
pagine: 303 - 308
SICI:
0262-0898(2000)18:4<303:ROTFPI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-FACTOR VIIA; PLASMINOGEN-ACTIVATOR RECEPTOR; SERINE-PROTEASE INHIBITOR; CDNA CLONING; IN-VITRO; EXPRESSION; CELLS; UROKINASE; METASTASIS; CANCER;
Keywords:
invasion; melanoma; metastasis; TFPI-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, 1 Illini Dr, Peoria, IL 61656 USA Univ Illinois 1 Illini Dr Peoria IL USA 61656 Peoria, IL 61656 USA
Citazione:
S.D. Konduri et al., "Role of tissue factor pathway inhibitor-2 (TFPI-2) in amelanotic melanoma (C-32) invasion", CLIN EXP M, 18(4), 2000, pp. 303-308

Abstract

Human tissue factor pathway inhibitor-2 (TFPI-2), also known as placental protein (PP5) and matrix-associated serine protease inhibitor (MSPI), is a 32-kDa extracellular matrix (ECM) protein consisting of three tandomly arranged Kunitz-type domains that inhibits plasmin, trypsin, chymotrypsin, cathepsin G and plasma kallikrein but not urokinase and tissue-type plasminogenactivators or thrombin. Earlier studies in our laboratory revealed that the production of TFPI-2 is reduced or absent during the tumor progression ofhuman gliomas. In the present study, we investigated the role of TFPI-2 inthe invasiveness of the amelanotic melanoma cell line C-32. We stably transfected C-32 cells with a vector capable of expressing TFPI-2 in a sense orientation (0.7 kb). TFPI-2 protein production was then determined by western blotting and the mRNA level by northern blotting in parental and stably transfected (vector and sense) clones. The levels of TFPI-2 protein and mRNAwere significantly higher in the sense clones, but neither was detected inparental and vector control clones. In addition, in vitro Matrigel invasion/migration assays revealed that the invasive behavior of sense clones was inhibited compared with the behavior of parental and vector clones. This isthe first study to show that the upregulation of TFPI-2 plays a significant role in reducing the invasive behavior of human amelanotic melanomas.

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Documento generato il 30/10/20 alle ore 08:48:18