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Titolo:
Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles
Autore:
Borges, R; Jaen, R; Freire, F; Gomez, JF; Villafruela, C; Yanes, E;
Indirizzi:
Univ La Laguna, Fac Med, Unidad Farmacol, Tenerife 38071, Spain Univ La Laguna Tenerife Spain 38071 idad Farmacol, Tenerife 38071, Spain Univ Las Palmas de Gran Canaria, Fac Med, Serv Microscopia Elect, Las Palmas Gran Canaria, Spain Univ Las Palmas de Gran Canaria Las Palmas Gran Canaria Spain ia, Spain
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 304, anno: 2001,
pagine: 159 - 164
SICI:
0302-766X(200104)304:1<159:MAFCOB>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEDIAK-HIGASHI-SYNDROME; CHROMAFFIN CELLS; EXOCYTOSIS; GRANULES; RELEASE; MAST; RAT; CAPACITANCE; FUSION;
Keywords:
amperometry; exocytosis; mast cells; Chediak-Higashi syndrome; microelectrodes; mouse, beige (C57BL/6N-bg);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Borges, R Univ La Laguna, Fac Med, Unidad Farmacol, Tenerife 38071, Spain Univ La Laguna Tenerife Spain 38071 col, Tenerife 38071, Spain
Citazione:
R. Borges et al., "Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles", CELL TIS RE, 304(1), 2001, pp. 159-164

Abstract

We tested whether the giant secretory granules observed in the mast cells of the naturally occurring mutant beige mouse (BM) (C57BL/6N-bg) were also present in the adrenal chromaffin cells. The presence of large chromaffin granules (CG) would be a valuable tool for the study of exocytosis in neuronal tissues. Conversely, the observation of large vesicles within chromaffincells that are different from CG could indicate that CC are of a differentorigin than granules of mast cells. Ultrastructural analysis demonstrated the presence of large lysososmal-like vesicles in the BM, and also a discrete increase in the number of CG with diameters larger than 240 nm but not of giant CC. In addition, amperometric measurements of single-event exocytosis, using carbon fiber microelectrodes, showed no differences between the quantal size of secretory events from BM and wildtype or bovine chromaffin cells. Minor but significant differences were found between the kinetics of exocytosis in SM cells and wild-type mouse cells. We conclude that CG, but not the abnormal-sized vesicles found in BM chromaffin cells contribute to the catecholamine secretion and that abnormal secretory granules are not present in adrenergic cell lineage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:56:49