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Titolo:
Quantitative analysis of epithelial cell aggregation in the simple metazoan Hydra reveals a switch from homotypic to heterotypic cell interactions
Autore:
Hobmayer, B; Snyder, P; Alt, D; Happel, CM; Holstein, TW;
Indirizzi:
Univ Technol, Inst Zool, Dept Mol Cell Biol, D-64287 Darmstadt, Germany Univ Technol Darmstadt Germany D-64287 Biol, D-64287 Darmstadt, Germany
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 304, anno: 2001,
pagine: 147 - 157
SICI:
0302-766X(200104)304:1<147:QAOECA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
REAGGREGATED CELLS; ADHESION MOLECULES; ENDODERMAL TISSUE; HEAD ACTIVATOR; MORPHOGENESIS; REGENERATION; DROSOPHILA; JUNCTIONS; CADHERINS; IDENTIFICATION;
Keywords:
cell sorting; cell adhesion; tissue formation; morphogenesis; evolution; Hydra magnipapillata (Cridaria);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hobmayer, B Univ Technol, Inst Zool, Dept Mol Cell Biol, Schnittspahnstr 10, D-64287 Darmstadt, Germany Univ Technol Schnittspahnstr 10 Darmstadt Germany D-64287 any
Citazione:
B. Hobmayer et al., "Quantitative analysis of epithelial cell aggregation in the simple metazoan Hydra reveals a switch from homotypic to heterotypic cell interactions", CELL TIS RE, 304(1), 2001, pp. 147-157

Abstract

Hydra, a member of the diploblastic phylum Cnidaria, exhibits the most basic type of organized metazoan tissues. Two unicellular sheets of polarized epithelial cells - ectoderm and endoderm - form a double layer throughout the body column. The double layer can be reestablished from single-cell suspensions by tissue-specific cell-sorting processes. However, the underlying pattern of interactions between ectodermal and endodermal epithelial cells responsible for double-layer formation is unclear, By analyzing cell interactions in a quantitative adhesion assay using mechanically dissociated Hydra epithelial cells, we show that aggregation proceeds in two steps. First, homotypic interactions within ectodermal epithelial cells (ecto-ecto) and within endodermal epithelial cells (endo-endo) form homotypic cell clusters. Second, at an aggregate size of about ten epithelial cells/cluster, ectodermal and endodermal clusters start to form heterotypic aggregates. Homotypic ecto-ecto inter actions are inhibited by a polyclonal anti-Hydra membraneantiserum, and under these conditions homotypic endo-endo interactions do not proceed beyond a size of about ten epithelial cells/cluster. These datasuggest that homotypic cell clusters reduce their initial homotypic affinity and acquire a new heterotypic affinity. A link between cell adhesion andcell signaling in early Hydra aggregates is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:08:42