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Titolo:
Activation of mitogen-activated protein kinase cascades is involved in regulation of bone morphogenetic protein-2-induced osteoblast differentiation in pluripotent C2Cl2 cells
Autore:
Gallea, S; Lallemand, F; Atfi, A; Rawadi, G; Ramez, V; Spinella-Jaegle, S; Kawai, S; Faucheu, C; Huet, L; Baron, R; Roman-Roman, S;
Indirizzi:
Hoechst Marion Roussel, Bone Dis Grp, F-93230 Romainville, France Hoechst Marion Roussel Romainville France F-93230 30 Romainville, France Hop St Antoine, INSERM U482, Paris, France Hop St Antoine Paris FranceHop St Antoine, INSERM U482, Paris, France Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA Yale Univ NewHaven CT USA 06510 Dept Cell Biol, New Haven, CT 06510 USA Yale Univ, Sch Med, Dept Orthoped, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 , Dept Orthoped, New Haven, CT 06510 USA
Titolo Testata:
BONE
fascicolo: 5, volume: 28, anno: 2001,
pagine: 491 - 498
SICI:
8756-3282(200105)28:5<491:AOMPKC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; N-TERMINAL KINASE; INHIBITS MYOGENIC DIFFERENTIATION; SIGNAL-TRANSDUCTION; C2C12 MYOBLASTS; MAP KINASE; PATHWAY; P38; EXPRESSION; GENE;
Keywords:
C2Cl2; bone morphogenetic protein (BMP)-2; MAPK; osteoblast differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Roman-Roman, S Hoechst Marion Roussel, Bone Dis Grp, 111 Route Noisy, F-93230 Romainville, France Hoechst Marion Roussel 111 Route Noisy RomainvilleFrance F-93230
Citazione:
S. Gallea et al., "Activation of mitogen-activated protein kinase cascades is involved in regulation of bone morphogenetic protein-2-induced osteoblast differentiation in pluripotent C2Cl2 cells", BONE, 28(5), 2001, pp. 491-498

Abstract

Bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta (TGF-beta) superfamily, is able to induce osteoblastic differentiation of C2C12 cells. Both Smad and mitogen-activated protein kinase (MAPK)pathways are essential components of the TGF-P superfamily signaling machinery. Although Smads have been demonstrated to participate in the BMP-2-induced osteoblastic differentiation of C2C12 cells, the role of MAPK has not been addressed. This report shows that BMP-2 activates ERK and p38, but notJNK, in C2C12 cells. Pretreatment of cells with the p38 inhibitor, SB203580, dramatically reduced BMP-2-induced expression of the osteoblast markers alkaline phosphatase (ALP) and osteocalcin (OC). Nevertheless, overexpression of MKK3, a protein kinase that phosphorylates and activates p38, failed to induce ALP or OC expression in the absence of BMP-2, indicating that p38activation is necessary but not sufficient for the acquisition of the osteoblast phenotype by these cells. Although ALP induction was increased slightly in the presence of PD-98059, a selective inhibitor of the ERK cascade, this compound significantly inhibited both steady-state and BMP-2-induced OC RNA levels. Our results indicate that p38 and ERK cascades play a crucialrole in the osteoblast differentiation of C2C12 cells mediated by BMP-2. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:14:20