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Titolo:
Tumor necrosis factor-alpha cooperates with receptor activator of nuclear factor kappa B ligand in generation of osteoclasts in stromal cell-depletedrat bone marrow cell culture
Autore:
Komine, M; Kukita, A; Kukita, T; Ogata, Y; Hotokebuchi, T; Kohashi, O;
Indirizzi:
Saga Med Sch, Dept Microbiol, Saga 8498501, Japan Saga Med Sch Saga Japan 8498501 Sch, Dept Microbiol, Saga 8498501, Japan Saga Med Sch, Dept Orthopaed, Saga 8498501, Japan Saga Med Sch Saga Japan 8498501 Sch, Dept Orthopaed, Saga 8498501, Japan Saga Med Sch, Dept Pediat, Saga 8498501, Japan Saga Med Sch Saga Japan 8498501 ed Sch, Dept Pediat, Saga 8498501, Japan Kyushu Univ, Fac Dent, Dept Anat 2, Fukuoka 812, Japan Kyushu Univ Fukuoka Japan 812 Fac Dent, Dept Anat 2, Fukuoka 812, Japan
Titolo Testata:
BONE
fascicolo: 5, volume: 28, anno: 2001,
pagine: 474 - 483
SICI:
8756-3282(200105)28:5<474:TNFCWR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION FACTOR; RHEUMATOID-ARTHRITIS; IN-VITRO; OSTEOPROTEGERIN LIGAND; CALCITONIN RECEPTOR; OSTEOBLASTIC CELLS; PROGENITOR CELLS; TNF RECEPTOR; FACTOR-BETA; T-CELLS;
Keywords:
osteoclast differentiation; tumor necrosis factor (TNF)-alpha; hematopoietic cell; receptor activator of nuclear factor kappa B ligand (RANKL); osteoclast differentiation factor (ODF); receptor activator of nuclear factor kappa B (RANK); osteoprotegerin (OPG);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Kukita, A Saga Med Sch, Dept Microbiol, 5-1-1 Nabeshima, Saga 8498501, Japan Saga Med Sch 5-1-1 Nabeshima Saga Japan 8498501 8498501, Japan
Citazione:
M. Komine et al., "Tumor necrosis factor-alpha cooperates with receptor activator of nuclear factor kappa B ligand in generation of osteoclasts in stromal cell-depletedrat bone marrow cell culture", BONE, 28(5), 2001, pp. 474-483

Abstract

A member of the tumor necrosis factor (TNF) family, receptor activator of nuclear factor kappaB ligand (RANKL; also known as ODF, OPGL, and TRANCE), plays critical roles in osteoclast differentiation and activation in the presence of macrophage colony-stimulating factor (M-CSF), Recently, TNF-alphahas also been shown to induce the formation of multinucleated osteoclast-like cells (MNCs) in the presence of M-CSF from mouse macrophages, We demonstrated that mononuclear preosteoclast-like cells (POCs) were formed in the presence of conditioned medium of osteoblastic cells in a rat bone marrow culture depleted of stromal cells. Using this culture system, in this study we examined whether TNF-alpha affects differentiation into POCs from hematopoietic progenitor cells. Human TNF-alpha (hTNF-alpha) markedly stimulated the formation of POCs, Moreover, a concentration as low as 0.005 ng/mL, of hTNF-alpha increased the level of mRNA for calcitonin receptor (CTR) and cathepsin-g of POCs, The POCs induced by hTNF-alpha formed MNCs, which showeddentine-resorbing activity after coculture with primary osteoblasts, Stimulation was observed after 24 h of treatment with hTNF-alpha. only on day 1 or day 2 of the culture. After 24 h of hTNF-alpha treatment, expression of the receptor activator of nuclear factor kappaB (RANK) mRNA was markedly increased, The addition of soluble RANKL (sRANKL) to the preformed POCs efficiently induced MNCs, Interestingly, treatment of bone marrow cells with hTNF-alpha and sRANKL synergistically augmented the formation of MNCs, This formation was abolished by the addition of human osteoprotegerin (hOPG). These results suggest that cooperation of TNF-alpha and RANKL is important for osteoclastogenesis, (C) 2001 by Elsevier Science Inc, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:59:25