Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A quantitative analysis of purinoceptor expression in the bladders of patients with symptomatic outlet obstruction
Autore:
OReilly, BA; Kosaka, AH; Chang, TK; Ford, APDW; Popert, R; McMahon, SB;
Indirizzi:
St Thomas Hosp, London SE1 7EH, England St Thomas Hosp London England SE1 7EH omas Hosp, London SE1 7EH, England Guys Hosp, London SE1 9RT, England Guys Hosp London England SE1 9RTGuys Hosp, London SE1 9RT, England Roche Biosci, Neurobiol Unit, Palo Alto, CA USA Roche Biosci Palo Alto CAUSA Biosci, Neurobiol Unit, Palo Alto, CA USA Univ London Kings Coll, Ctr Res Neurosci, London, England Univ London Kings Coll London England Ctr Res Neurosci, London, England
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 7, volume: 87, anno: 2001,
pagine: 617 - 622
SICI:
1464-4096(200105)87:7<617:AQAOPE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERIATRIC VOIDING DYSFUNCTION; SMOOTH-MUSCLE CELLS; URINARY-BLADDER; OVERACTIVE BLADDER; STRUCTURAL BASIS; P2X RECEPTORS; VAS-DEFERENS; PCR; CALPONIN; RAT;
Keywords:
P2X receptor; PCR; detrusor. ATP; bladder nutlet obstruction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: O'Reilly, BA St Thomas Hosp, 6th Floor,North Wing,Lambeth Palace Rd, London SE1 7EH, England St Thomas Hosp 6th Floor,North Wing,Lambeth Palace Rd London England SE1 7EH
Citazione:
B.A. O'Reilly et al., "A quantitative analysis of purinoceptor expression in the bladders of patients with symptomatic outlet obstruction", BJU INT, 87(7), 2001, pp. 617-622

Abstract

Objective To compare the expression of the seven known P2X receptors in human bladder from male patients with detrusor instability caused by symptomatic bladder outlet obstruction with that from control bladders, using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. Patients and methods Real-time quantitative RT-PCR provides a system for detecting and analysing RNA. Bladder biopsies were obtained from nine patients undergoing prostate surgery and control biopsies were obtained From eight age-matched men undergoing routine bladder endoscopy studies, and who were asymptomatic. Total RNA was extracted from each sample and 10 ng of this used for individual PCR reactions. The expression levels of the seven P2X genes in the total RNA were then determined. Results In the control bladder, P2X(1) was by far the predominant purinergic receptor at the RNA level, the remainder consistently present in the order P2X(1) >> P2X(4) > P2X(2) >P2X(7) > P2X(5) >> P2X(3) = P2X(6) = 0. Calponin, a smooth muscle-specific protein, was used as a marker for smooth muscle content. In bladder from symptomatic patients. the P2X(1)/ calponin ratio was greater than that in controls (P=0.016). There appeared to be no difference in P2X(2), but P2X(4), P2X(5), and P2X(7) were all greater in the symptomatic bladder than in the controls. although these differences were notsignificant. Conclusion P2X(1) is the predominant purinoceptor subtype in the human male bladder, consistent with pharmacological evidence. The amount of P2X(1) receptor per smooth muscle cell is greater in the obstructed than in controlbladder, suggesting an increase in purinergic function in the unstable bladder arising from bladder outlet obstruction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 05:38:03