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Titolo:
An oxidized derivative of cholesterol increases the release of soluble vascular cell adhesion molecule-1 from human umbilical vein endothelial cells in culture
Autore:
Tamasawa, N; Murakami, H; Matsui, J; Yamato, K; Guan, JZ; Imaizumi, T; Fujimoto, K; Yoshida, H; Satoh, K; Suda, T;
Indirizzi:
Hirosaki Univ, Sch Med, Dept Internal Med 3, Hirosaki, Aomori 0368562, Japan Hirosaki Univ Hirosaki Aomori Japan 0368562 rosaki, Aomori 0368562, Japan Hirosaki Univ, Sch Med, Dept Vasc Biol, Hirosaki, Aomori 0368562, Japan Hirosaki Univ Hirosaki Aomori Japan 0368562 rosaki, Aomori 0368562, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 3, volume: 1531, anno: 2001,
pagine: 178 - 187
SICI:
1388-1981(20010430)1531:3<178:AODOCI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-ACTIVATING-FACTOR; LOW-DENSITY LIPOPROTEINS; SMOOTH-MUSCLE CELLS; 7-KETOCHOLESTEROL; EXPRESSION; ATHEROSCLEROSIS; OXYSTEROLS; LDL; APOPTOSIS; LIPIDS;
Keywords:
7-ketocholesterol; endothelial cell; vascular cell adhesion molecule-1; tumor necrosis factor-alpha; oxidized low density lipoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Tamasawa, N Hirosaki Univ, Sch Med, Dept Internal Med 3, 5 Zaifucho, Hirosaki, Aomori 0368562, Japan Hirosaki Univ 5 Zaifucho Hirosaki Aomori Japan 0368562 , Japan
Citazione:
N. Tamasawa et al., "An oxidized derivative of cholesterol increases the release of soluble vascular cell adhesion molecule-1 from human umbilical vein endothelial cells in culture", BBA-MOL C B, 1531(3), 2001, pp. 178-187

Abstract

Treatment of human umbilical vein endothelial cells (HUVECs) with 7-ketocholesterol resulted in an increased release of soluble vascular cell adhesion molecule-1 (VCAM-1) into culture medium. 7-Ketocholesterol did not enhance the expression of mRNA for VCAM-1. 7 beta -Hydroxy- or 25-hydroxycholesterol had no effect on soluble VCAM-1 levels. Western blot analysis revealed that soluble VCAM-1, in the conditioned medium of both 7-ketocholesterol-stimulated and control cells, had a molecular size of 100 kDa. Stimulation ofthe TNF-alpha -treated HUVECs with 7-ketocholesterol further increased thelevels of soluble VCAM-1 in the culture medium. Again, 7-ketocholesterol did not affect the VCAM-1 mRNA level, which was enhanced by TNF-alpha. Pretreatment of the cells with tissue inhibitor of membrane metalloproteinase-2 (TIMP-2) completely inhibited the release of VCAM-1 in response to 7-ketocholesterol but TIMP-1 had no effect. Adherence of mononuclear cells to TNF-stimulated HUVEC monolayers was slightly inhibited by 7-ketocholesterol, butthis oxysterol did not affect the basal adherence to non-stimulated HUVECs. Immunofluorescent staining of the cells confirmed diffuse perinuclear distribution of VCAM-1 in HUVECs treated with TNF-alpha, but 7-ketocholesteroldid not affect the intensity or distribution of immunofluorescence. We conclude that 7-ketocholesterol releases VCAM-1 from the endothelium probably by a proteolytic process. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:24:53