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Titolo:
Tissue-specific activity of lipoprotein lipase in skeletal muscle regulates the expression of uncoupling protein 3 in transgenic mouse models
Autore:
Kratky, D; Strauss, JG; Zechner, R;
Indirizzi:
Karl Franzens Univ Graz, Inst Mol Biol Biochem & Microbiol, A-8010 Graz, Austria Karl Franzens Univ Graz Graz Austria A-8010 robiol, A-8010 Graz, Austria
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 355, anno: 2001,
parte:, 3
pagine: 647 - 652
SICI:
0264-6021(20010501)355:<647:TAOLLI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN ADIPOSE-TISSUE; HIGH-DENSITY-LIPOPROTEIN; FREE FATTY-ACIDS; MESSENGER-RNA EXPRESSION; GENE-EXPRESSION; NONSHIVERING THERMOGENESIS; CHOLESTEROL LEVELS; THYROID-HORMONE; KNOCKOUT MICE; UP-REGULATION;
Keywords:
brown adipose tissue; energy metabolism; induced mutant mice; non-esterified fatty acids; thermogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Zechner, R Karl Franzens Univ Graz, Inst Mol Biol Biochem & Microbiol, Heinrichstr 31A, A-8010 Graz, Austria Karl Franzens Univ Graz Heinrichstr 31A Graz Austria A-8010 ia
Citazione:
D. Kratky et al., "Tissue-specific activity of lipoprotein lipase in skeletal muscle regulates the expression of uncoupling protein 3 in transgenic mouse models", BIOCHEM J, 355, 2001, pp. 647-652

Abstract

Uncoupling protein (UCP)-2 and UCP-3 an two recently discovered proteins similar to UCP-1, which regulates thermogenesis in brown adipose tissue (BAT). Whereas UCP-1 expression is restricted to BAT, UCP-2 is widely expressed. UCP-3 is found mainly in skeletal muscle and BAT. A large body of evidence exists that the expression of UCP-2 and UCP-3 in skeletal muscle of mice is regulated by feediug/fasting, and some studies have suggested that this effect might be caused by the changing concentration of plasma non-esterified fatty acids (NEFAs). In an attempt to determine whether the increased import of triacylglycerol-derived NEFAs can also affect UCP expression, we determined the mRNA levels of UCP-1, UCP-2 and UCP-3 in BAT and muscle of induced mutant mouse lines that overexpressed or lacked lipoprotein lipase (LPL) in these tissues. The expression levels of UCP-1 and UCP-2 in BAT and inskeletal and cardiac muscle respectively were not affected by variations in tissue LPL activities. In contrast, UCP-3 mRNA levels were induced 3.4-fold in mice with high levels of LPL in skeletal muscle, and down-regulated in mice that lacked LPL in skeletal muscle. The presence or absence of LPL in BAT had no effect on UCP-3 expression levels. The response of UCP-3 mRNA expression to variations in LPL activity in skeletal muscle was independentof the feeding status or of plasma NEFA concentrations. These findings indicated that NEFAs as lipolytic products of LPL-mediated triacylglycerol hydrolysis markedly affect UCP-3 expression and that increased LPL activities occurring during fasting in skeletal muscle contribute to the induction of UCP-3 expression by promoting the increased uptake of NEFAs. In addition, our results demonstrate that UCP-2 and UCP-3 are differentially regulated inresponse to LPL-mediated NEFA uptake in skeletal muscle of mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:36:27