Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulation of mouse DNA topoisomerase III alpha gene expression by YY1 andUSF transcription factors
Autore:
Park, EJ; Han, SY; Chung, IK;
Indirizzi:
Yonsei Univ, Coll Sci, Prot Network Res Ctr, Dept Biol, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 ept Biol, Seoul 120749, South Korea
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 283, anno: 2001,
pagine: 384 - 391
SICI:
0006-291X(20010504)283:2<384:ROMDTI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR LATE PROMOTER; RNA POLYMERASE-II; UPSTREAM ELEMENT; BINDING PROTEIN; INITIATION; CLONING; REGION; RECOMBINATION; MECHANISM; ISLANDS;
Keywords:
topoisomerase III; mouse; gene expression; promoter; transcription; YY1; USF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, IK Yonsei Univ, Coll Sci, Prot Network Res Ctr, Dept Biol, 134 Shinchon Dong,Seoul 120749, South Korea Yonsei Univ 134 Shinchon Dong Seoul South Korea 120749 th Korea
Citazione:
E.J. Park et al., "Regulation of mouse DNA topoisomerase III alpha gene expression by YY1 andUSF transcription factors", BIOC BIOP R, 283(2), 2001, pp. 384-391

Abstract

To investigate the mechanisms responsible for the regulation of DNA topoisomerase III alpha (TOP3 alpha) gene expression, the promoter region of the mouse gene has been cloned and analyzed. The promoter region is moderately high in GC content and lacks a canonical TATA box, typical for promoters ofa number of housekeeping genes. Transient expression of a luciferase reporter gene under the control of serially deleted 5'-flanking sequences demonstrated that the 34-bp region from -137 to -170 upstream of the transcription initiation site contains a positive regulatory element(s) for the efficient expression of mouse TOP3 alpha gene. Combined analyses by gel mobility shift and supershift assays revealed that both YY1 and USF transcription factors were capable of binding to the 34-bp region. When YY1 and USF-binding elements were selectively mutated, the luciferase activity of the resulted constructs was greatly reduced, indicating that both YY1 and USF function as transcriptional activators. Interestingly, YY1 and USF-binding elements are conserved in both human and mouse TOP3 alpha promoters. This suggests that mammalian TOP3 alpha genes may possess a common mechanism of transcription regulation through these elements. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:34:07