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Titolo:
Upregulatory expression of furin and transforming growth factor-beta by fluid shear stress in vascular endothelial cells
Autore:
Negishi, M; Lu, DH; Zhang, YQ; Sawada, Y; Sasaki, T; Kayo, T; Ando, J; Izumi, T; Kurabayashi, M; Kojima, I; Masuda, H; Takeuchi, T;
Indirizzi:
Gunma Univ, Inst Mol & Cellular Regulat, Dept Mol Med, Maebashi, Gumma 3718512, Japan Gunma Univ Maebashi Gumma Japan 3718512 d, Maebashi, Gumma 3718512, Japan Gunma Univ, Inst Mol & Cellular Regulat, Dept Cell Biol, Maebashi, Gumma 3718512, Japan Gunma Univ Maebashi Gumma Japan 3718512 l, Maebashi, Gumma 3718512, Japan Gunma Univ, Sch Med, Dept Internal Med 2, Maebashi, Gumma 371, Japan GunmaUniv Maebashi Gumma Japan 371 nal Med 2, Maebashi, Gumma 371, Japan Univ Tokyo, Grad Sch Med, Dept Biomed Engn, Tokyo, Japan Univ Tokyo Tokyo Japan yo, Grad Sch Med, Dept Biomed Engn, Tokyo, Japan Akita Univ, Sch Med, Dept Pathol, Akita 010, Japan Akita Univ Akita Japan 010 Univ, Sch Med, Dept Pathol, Akita 010, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 5, volume: 21, anno: 2001,
pagine: 785 - 790
SICI:
1079-5642(200105)21:5<785:UEOFAT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROCESSING ENDOPROTEASE FURIN; NATRIURETIC PEPTIDE; PLASMINOGEN-ACTIVATOR; GENE-EXPRESSION; II RECEPTOR; IN-VIVO; GROWTH-FACTOR-BETA-1; TGF-BETA-1; FLOW; FACTOR-BETA(1);
Keywords:
transforming growth factor-beta; furin; endoprotease; shear stress; endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Takeuchi, T Gunma Univ, Inst Mol & Cellular Regulat, Dept Mol Med, Maebashi, Gumma 3718512, Japan Gunma Univ Maebashi Gumma Japan 3718512 Gumma 3718512, Japan
Citazione:
M. Negishi et al., "Upregulatory expression of furin and transforming growth factor-beta by fluid shear stress in vascular endothelial cells", ART THROM V, 21(5), 2001, pp. 785-790

Abstract

Furin, a yeast Kex2-family endoprotease, converts many vasoregulatory propeptides, including pro-transforming growth factor (TGF)-beta to their mature forms. We examined whether furin expression is regulated by shear stress in vivo and in vitro. When an arteriovenous shunt was placed between the carotid artery and external jugular vein in rabbits, furin and TGF-beta were highly expressed in shear stress-loaded endothelial cells. Exposure of bovine aortic endothelial cells in culture to shear stress induced furin and TGF-beta expression in a similar manner. Molecular analysis of furin expression in bovine aortic endothelial cells revealed that shear stress increases the furin gene expression at transcriptional levels. Furthermore, TGF-beta itself increased the furin mRNA levels. Shear-mediated furin expression waspartly mediated by TGF-beta because shear-induced furin mRNA levels were considerably decreased by overexpression of the truncated form of the TGF-beta type II receptor. Likewise, blockade of furin activity by a furin inhibitor significantly decreased the endothelial production of mature TGF-beta. Taken together, the results indicate that furin expression is induced and maintained by a coordination of shear stress and TGF-beta. Increased furin expression may facilitate the formation of mature TGF-beta, resulting in theenhanced effects of TGF-beta on endothelial cells and vascular smooth muscle cells in the vasculature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:50:13