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Titolo:
Insulin-like growth factor-II in endocrine pancreatic tumours - Immmunohistochemical, biochemical and in situ hybridization findings
Autore:
Hoog, A; Kjellman, M; Nordqvist, ACS; Hoog, CM; Juhlin, C; Falkmer, S; Schalling, M; Grimelius, L;
Indirizzi:
Karolinska Hosp & Inst, Tumour Pathol Unit, Dept Pathol & Oncol, Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden thol & Oncol, Stockholm, Sweden Karolinska Hosp & Inst, Dept Surg, Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden t, Dept Surg, Stockholm, Sweden Karolinska Hosp & Inst, Dept Clin Neurosci, Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden lin Neurosci, Stockholm, Sweden Karolinska Hosp & Inst, Dept Mol Med, Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden Dept Mol Med, Stockholm, Sweden Norwegian Univ Sci & Technol, Inst Lab Med, N-7034 Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway N-7034 4 Trondheim, Norway Univ Uppsala Hosp, Dept Surg, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 t Surg, S-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Genet & Pathol, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 Pathol, S-75185 Uppsala, Sweden
Titolo Testata:
APMIS
fascicolo: 2, volume: 109, anno: 2001,
pagine: 127 - 140
SICI:
0903-4641(200102)109:2<127:IGFIEP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURON-SPECIFIC ENOLASE; IGF-BINDING-PROTEINS; MAJOR ISLET HORMONES; HUMAN FETAL PANCREAS; IMMUNOHISTOCHEMICAL LOCALIZATION; MONOCLONAL-ANTIBODIES; MESSENGER-RNA; EXPRESSION; CELLS; TUMORS;
Keywords:
insulin-like growth factor II (IGF-II); endocrine pancreatic tumour; insulinoma; immunohistochemistry; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Hoog, A Karolinska Hosp, Dept Pathol & Oncol, Tumour Pathol Unit, SE-17176Stockholm, Sweden Karolinska Hosp Stockholm Sweden SE-17176 7176 Stockholm, Sweden
Citazione:
A. Hoog et al., "Insulin-like growth factor-II in endocrine pancreatic tumours - Immmunohistochemical, biochemical and in situ hybridization findings", APMIS, 109(2), 2001, pp. 127-140

Abstract

In earlier studies a high-molecular-weight (HMW) insulin-like,growth factor-II (IGF-II) peptide was identified in adult human pancreas and localized to the insulin-producing B-cells. This peptide has now been investigated inneoplastic insulin cells. Forty endocrine pancreatic tumours and 17 pancreatic adenocarcinomas of ductal type were included in the study. All cases were investigated with immunohistochemical techniques using antibodies to IGF-II, insulin, pro-insulin, glucagon, somatostatin, pancreatic polypeptide,gastrin and vasoactive intestinal peptide (VIP). Frozen tissue from nine tumours and two normal pancreatic glands was extracted, gel separated, and quantified using radioimmunoassay. The tumours were also investigated by in situ hybridization. IGF-II-immunoreactive cells were found in nearly all the 18 insulin-producing tumours (16/18), in a minority of the other endocrine tumours, but not in pancreatic adenocarcinomas. All extracts from the endocrine tumours showed varying amounts of IGF-II and had different molecular-weight forms. The immunohistochemical and radioimmunoassay findings are both based on immunological binding and were further confirmed by Northern blot and in situ hybridization. These results show that IGF-II is expressed in insulin-producing tumours as well as in pancreatic tumours producing other peptides, in contrast to normal pancreatic islets where IGF-II is found exclusively in insulin-producing cells.

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Documento generato il 30/05/20 alle ore 14:55:26