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Titolo:
IDENTIFICATION OF FUNCTIONAL ELEMENTS IN THE PROMOTER REGION OF THE HUMAN GENE FOR THYMIDYLATE SYNTHASE AND NUCLEAR FACTORS THAT REGULATE THE EXPRESSION OF THE GENE
Autore:
HORIE N; TAKEISHI K;
Indirizzi:
UNIV SHIZUOKA,SCH FOOD & NUTR SCI,52-1 YADA SHIZUOKA 422 JAPAN UNIV SHIZUOKA,SCH FOOD & NUTR SCI SHIZUOKA 422 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 29, volume: 272, anno: 1997,
pagine: 18375 - 18381
SICI:
0021-9258(1997)272:29<18375:IOFEIT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE FM3A CELLS; TRANSCRIPTION FACTOR; NEGATIVE MUTANTS; CACCC ELEMENT; SEQUENCES; BETA; COOPERATION; CHROMOSOME; INDUCTION; UPSTREAM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
N. Horie e K. Takeishi, "IDENTIFICATION OF FUNCTIONAL ELEMENTS IN THE PROMOTER REGION OF THE HUMAN GENE FOR THYMIDYLATE SYNTHASE AND NUCLEAR FACTORS THAT REGULATE THE EXPRESSION OF THE GENE", The Journal of biological chemistry, 272(29), 1997, pp. 18375-18381

Abstract

To identify the essential motifs of the promoter of the human gene for thymidylate synthase (TS), we constructed a set of deletion mutants from the 5'-terminal region of the human TS gene, From the results of assays of the expression of chloramphenicol acetyltransferase (CAT), we identified two functional elements with positive effects on the promoter activity: a CACCC box (CCACACCC) and an Spl-binding motif (GAGGCGGA) that was homologous to the Spl binding site in the mouse TS gene, In addition, negative regulatory sequences were identified between thetwo positive elements and in the region upstream of the CACCC box, The results of gel mobility shift analyses suggested that Spl binds to the Spl-binding motif of the human TS gene promoter and that multiple nuclear factors that are related to Spl bind to the CACCC box, Furthermore, the binding of Spl to mutated Spl-binding motifs in the promoter region of the human TS gene was correlated with the promoter activity,as measured by the CAT assay, Therefore, the Spl motif seems to be a major contributor to the basic promoter activity of the human TS gene,although multiple positive and negative regulatory elements are involved in the regulated expression of this gene.

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Documento generato il 01/10/20 alle ore 15:11:15