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Titolo:
Role of chloride in constriction of descending vasa recta by angiotensin II
Autore:
Zhang, Z; Huang, JMC; Turner, MR; Rhinehart, KL; Pallone, TL;
Indirizzi:
Univ Maryland, Div Nephrol, Sch Med, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 ol, Sch Med, Baltimore, MD 21201 USA Univ Leicester, Inst Cardiovasc Res, Leicester LE1 7RH, Leics, England Univ Leicester Leicester Leics England LE1 7RH er LE1 7RH, Leics, England
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
fascicolo: 6, volume: 280, anno: 2001,
pagine: R1878 - R1886
SICI:
0363-6119(200106)280:6<R1878:ROCICO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; ENDOTHELIAL-CELLS; RESISTANCE ARTERIES; BLOOD-FLOW; CHANNELS; MICROVESSELS; MODULATION; CURRENTS; ACID;
Keywords:
medulla; kidney; microcirculation; membrane potential; patch clamp; niflumic acid; indanyloxyacetic acid 94;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Pallone, TL Univ Maryland, Div Nephrol, Sch Med, N3W143,22 S Greene St, Baltimore, MD 21201 USA Univ Maryland N3W143,22 S Greene St Baltimore MD USA 21201 USA
Citazione:
Z. Zhang et al., "Role of chloride in constriction of descending vasa recta by angiotensin II", AM J P-REG, 280(6), 2001, pp. R1878-R1886

Abstract

We investigated the dependence of ANG II (10(-8) M)-induced constriction of outer medullary descending vasa recta (OMDVR) on membrane potential (Cm) and chloride ion. ANG II depolarized OMDVR, as measured by fully loading them with the voltage-sensitive dye bis[1,3-dibutylbarbituric acid-(5)] trimethineoxonol [DiBAC(4)(3)] or selectively loading their pericytes. ANG II was also observed to depolarize pericytes from a resting value of -55.6 +/- 2.6 to -26.2 +/- 5.4 mV when measured with gramicidin D-perforated patches. When measured with DiBAC4(3) in unstimulated vessels, neither changing extracellular Cl- concentration ([Cl-]) nor exposure to the chloride channel blocker indanyloxyacetic acid 94 (IAA-94; 30 muM) affected Psim. In contrast,IAA-94 repolarized OMDVR pretreated with ANG II. Neither IAA-94 (30 muM) nor niflumic acid (30 muM, 1 mM) affected the vasoactivity of unstimulated OMDVR, whereas both dilated ANG II-preconstricted vessels. Reduction of extracellular [Cl-] from 150 to 30 meq/l enhanced ANG II-induced constriction. Finally, we identified a Cl- channel in OMDVR pericytes that is activated by ANG II or by excision into extracellular buffer. We conclude that constriction of OMDVR by ANG II involves pericyte depolarization due, in part, to increased activity of chloride channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:11:12