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Titolo:
Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12
Autore:
Maeyama, T; Kuwano, K; Kawasaki, M; Kunitake, R; Hagimoto, N; Hara, N;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Res Inst Dis Chest, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 st, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 6, volume: 280, anno: 2001,
pagine: L1128 - L1137
SICI:
1040-0605(200106)280:6<L1128:AOBPIM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY FIBROSIS; STIMULATORY FACTOR INTERLEUKIN-12; INTERFERON-GAMMA; IMMUNE-RESPONSES; P40 SUBUNIT; LOCAL PRODUCTION; LUNG FIBROSIS; IN-VIVO; IL-12; CELLS;
Keywords:
T helper type 1 cell; T helper type 2 cell; Fas-Fas ligand pathway; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kuwano, K Kyushu Univ, Grad Sch Med Sci, Res Inst Dis Chest, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 8128582, Japan
Citazione:
T. Maeyama et al., "Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12", AM J P-LUNG, 280(6), 2001, pp. L1128-L1137

Abstract

We previously demonstrated essential roles of the Fas-Fas ligand (FasL) pathway in bleomycin-induced pneumopathy in mice. T lymphocytes and natural killer cells express FasL on activation and use it as a cytotoxic effector molecule. Because interleukin (IL)-12 is known to play a critical role in cell-mediated immunity, we investigated whether anti-IL-12 antibody treatmentsuppresses the development of this model. The anti-IL-12 antibody treatment decreased the number of apoptotic cells and the degree of inflammation and fibrosis in lung tissue. The results of RT-PCR showed that IL-12p40, IL-12 receptor (R) beta2, interferon-gamma, tumor necrosis factor-alpha and FasL mRNAs were upregulated after bleomycin instillation. The upregulation of FasL, IL-12R beta2, and tumor necrosis factor-alpha mRNA expression in lungtissue was suppressed by anti-IL-12 antibody treatment. The results of enzyme-linked immunosorbent assay showed that the levels of IL-12p40, but not of IL-12p70, were increased in lung tissue after bleomycin instillation. Although the increase in IL-12R beta2 mRNA levels suggests that the T helper type 1 cell response may participate in lung injury, the increase in IL-12p40 supports T helper type 2 cell predominance in the fibrotic process of this model. The administration of anti-IL-12 antibody could be a novel therapy against lung injury and pulmonary fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:09:53