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Titolo:
Heterocellular gap junctional communication between alveolar epithelial cells
Autore:
Abraham, V; Chou, ML; George, P; Pooler, P; Zaman, A; Savani, RC; Koval, M;
Indirizzi:
Univ Penn, Sch Med, Inst Environm Med, Dept Physiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Physiol, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Inst Environm Med, Dept Pediat, Philadelphia, PA 19104USA Univ Penn Philadelphia PA USA 19104 ept Pediat, Philadelphia, PA 19104USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 6, volume: 280, anno: 2001,
pagine: L1085 - L1093
SICI:
1040-0605(200106)280:6<L1085:HGJCBA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY FIBROSIS; INDUCED LUNG INJURY; KERATINOCYTE GROWTH-FACTOR; INTERCELLULAR COMMUNICATION; SELECTIVE INTERACTIONS; RAT LUNG; EXPRESSION; CONNEXINS; CHANNELS; PROTEIN;
Keywords:
bleomycin; cell junctions; differentiation; connexin; cell-cell interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Koval, M Univ Penn, Sch Med, Inst Environm Med, Dept Physiol, 1 John Morgan Bldg,3620 Hamilton Walk, Philadelphia, PA 19104 USA Univ Penn 1 John Morgan Bldg,3620 Hamilton Walk Philadelphia PA USA 19104
Citazione:
V. Abraham et al., "Heterocellular gap junctional communication between alveolar epithelial cells", AM J P-LUNG, 280(6), 2001, pp. L1085-L1093

Abstract

We analyzed the pattern of gap junction protein (connexin) expression in vivo by indirect immunofluorescence. In normal rat lung sections, connexin (Cx)32 was expressed by type II cells, whereas Cx43 was more ubiquitously expressed and Cx46 was expressed by occasional alveolar epithelial cells. In response to bleomycin-induced lung injury, Cx46 was upregulated by alveolarepithelial cells, whereas Cx32 and Cx43 expression were largely unchanged. Given that Cx46 may form gap junction channels with either Cx43 or Cx32, we examined the ability of primary alveolar epithelial cells cultured for 6 days, which express Cx43 and Cx46, to form heterocellular gap junctions with cells expressing other connexins. Day 6 alveolar epithelial cells formed functional gap junctions with other day 6 cells or with HeLa cells transfected with Cx43 (HeLa/Cx43), but they did not communicate with HeLa/Cx32 cells. Furthermore, day 6 alveolar epithelial cells formed functional gap junction channels with freshly isolated type II cells. Taken together, these data are consistent with the notion that type I and type II alveolar epithelial cells communicate through gap junctions compatible with Cx43.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:40:55