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Titolo:
Regulation of ClC-2 chloride channels in T84 cells by TGF-alpha
Autore:
Bali, MZ; Lipecka, J; Edelman, A; Fritsch, J;
Indirizzi:
Fac Med Necker, INSERM, U467, F-75730 Paris 15, France Fac Med Necker Paris France 15 r, INSERM, U467, F-75730 Paris 15, France Hop St Vincent de Paul, CNRS, UPR 1524, F-75674 Paris, France Hop St Vincent de Paul Paris France F-75674 1524, F-75674 Paris, France
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 280, anno: 2001,
pagine: C1588 - C1598
SICI:
0363-6143(200106)280:6<C1588:ROCCCI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; PHOSPHOLIPASE C-GAMMA; PHOSPHATIDYLINOSITOL 3-KINASE; VOLUME DECREASE; POSTSYNAPTIC INHIBITION; POTASSIUM CHANNEL; EPITHELIAL-CELLS; NA+/H+ EXCHANGE; EGF RECEPTOR; RAT-BRAIN;
Keywords:
hydrogen ion concentration; protein kinase C; signal transduction; patch-clamp techniques;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Edelman, A Fac Med Necker, INSERM, U467, 156 Rue Vaugirard, F-75015 Paris,France Fac Med Necker 156 Rue Vaugirard Paris France F-75015 , France
Citazione:
M.Z. Bali et al., "Regulation of ClC-2 chloride channels in T84 cells by TGF-alpha", AM J P-CELL, 280(6), 2001, pp. C1588-C1598

Abstract

The almost ubiquitously expressed ClC-2 chloride channel is activated by hyperpolarization and osmotic cell swelling. Osmotic swelling also activatesa different class of outwardly rectifying chloride channels, and several reports point to a link between protein tyrosine phosphorylation and activation of these channels. This study examines the possibility that transforming growth factor-alpha (TGF-alpha) modulates ClC-2 activity in human colonicepithelial (T84) cells. TGF-alpha (0.17 nM) irreversibly inhibited ClC-2 current in nystatin-perforated whole cell patch-clamp experiments, whereas asuperimposed reversible activation of the current was observed at 8.3 nM TGF-alpha. Both effects required activation of the intrinsic epidermal growth factor receptor (EGFR) tyrosine kinase activity, of phosphoinositide 3-kinase, and of protein kinase C. With microspectrofluorimetry of the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, TGF-alpha was shown to reversibly alkalinize T84 cells at 8.3 nM but not at 0.17 nM, suggesting that 8.3 nM TGF-alpha -induced alkalinization activates ClC-2 current. This study indicates that ClC-2 channels are targets for EGFRsignaling in epithelial cells.

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Documento generato il 27/01/20 alle ore 01:16:24